高质量的胶孢炭疽菌 DSM102798 基因组组装和注释。

A high-quality genome assembly and annotation of Thielaviopsis punctulata DSM102798.

机构信息

Department of Biology, College of Science, United Arab Emirates University, Al Ain, 15551, United Arab Emirates.

Khalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, Al Ain, 15551, United Arab Emirates.

出版信息

Sci Data. 2024 Jul 9;11(1):745. doi: 10.1038/s41597-024-03458-y.

DOI:10.1038/s41597-024-03458-y

PMID:38982096

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11233662/

Abstract

Black scorch disease (BSD), caused by the fungal pathogen Thielaviopsis punctulata (Tp) DSM102798, poses a significant threat to date palm cultivation in the United Arab Emirates (UAE). In this study, Chicago and Hi-C libraries were prepared as input for the Dovetail HiRise pipeline to scaffold the genome of Tp DSM102798. We generated an assembly with a total length of 28.23 Mb comprising 1,256 scaffolds, and the assembly had a contig N50 of 18.56 kb, L50 of three, and a BUSCO completeness score of 98.6% for 758 orthologous genes. Annotation of this assembly produced 7,169 genes and 3,501 Gene Ontology (GO) terms. Compared to five other Thielaviopsis genomes, Tp DSM102798 exhibited the highest continuity with a cumulative size of 27.598 Mb for the first seven scaffolds, surpassing the assemblies of all examined strains. These findings offer a foundation for targeted strategies that enhance date palm resistance against BSD, and foster more sustainable and resilient agricultural systems.

摘要

黑腐病（BSD）由真菌病原体 Thielaviopsis punctulata（Tp）DSM102798 引起，对阿联酋（UAE）的棕榈树种植构成了重大威胁。在这项研究中，我们制备了芝加哥和 Hi-C 文库，作为 Dovetail HiRise 管道的输入，以构建 Tp DSM102798 的基因组支架。我们生成了一个总长度为 28.23 Mb 的组装体，包含 1,256 个支架，组装体的 contig N50 为 18.56 kb，L50 为 3，758 个同源基因的 BUSCO 完整性评分为 98.6%。该组装体的注释生成了 7,169 个基因和 3,501 个基因本体论（GO）术语。与其他五个 Thielaviopsis 基因组相比，Tp DSM102798 在前七个支架中表现出最高的连续性，累积大小为 27.598 Mb，超过了所有检查菌株的组装体。这些发现为针对 BSD 提高油棕抗性的靶向策略提供了基础，并促进了更可持续和有弹性的农业系统。