Pathmanathan Priya, Gomathi A, Ramesh Asha, Subrahmanyam Ch
Department of Chemistry, Mahindra University Hyderabad-500043 India
Department of Chemistry, Indian Institute of Technology Hyderabad-502285 India.
RSC Adv. 2024 Jul 9;14(30):21808-21820. doi: 10.1039/d4ra03559c. eCollection 2024 Jul 5.
Among the 3d-transition metal hydroxide series, nickel hydroxide is a well-studied electroactive catalyst. In particular, nickel hydroxide and its composite materials are well-suited for non-enzymatic glucose sensing. The electrocatalytic efficiency of nickel hydroxide is attributed to the thickness or to be precise, the thinness of the electroactive layer. Herein, we have successfully prepared metallic nickel@nickel hydroxide nanosheets through a straightforward one-pot solvothermal method. We were able to electrochemically generate a highly sensitive α-Ni(OH) on the nanosheets. The dynamic generation and synergy between α- and β-Ni(OH), imparts a glucose oxidase enzyme-like ability to the catalyst. Our proposed nickel nanozyme exhibits a good sensitivity of 683 μA mM cm for glucose. The sensor operates in the range of 0.001-3.1 mM, with a lower limit of detection (LOD) of 9.1 μM and exhibits a response time of ≈00.1 s. Nickel-nanozyme demonstrated better selectivity for glucose in the presence of interfering compounds. Notably, the sensor does not suffer from an interfering oxygen evolution reaction. This greatly improves sensitivity in glucose detection in lower concentrations making the sensor viable to measure salivary glucose levels. In this study, we demonstrate that our sensor can detect glucose in human saliva. The real sample analysis was carried out with saliva samples from three healthy human volunteers and one prediabetic volunteer. Our proposed sensor measurements show excellent agreement with calculated salivary glucose levels with 98% accuracy in sensing glucose in real saliva samples.
在3d过渡金属氢氧化物系列中，氢氧化镍是一种经过充分研究的电活性催化剂。特别是，氢氧化镍及其复合材料非常适合非酶葡萄糖传感。氢氧化镍的电催化效率归因于电活性层的厚度，或者更准确地说，是其薄度。在此，我们通过一种简单的一锅溶剂热法成功制备了金属镍@氢氧化镍纳米片。我们能够在纳米片上电化学生成高灵敏度的α-Ni(OH)。α-和β-Ni(OH)之间的动态生成和协同作用赋予了催化剂类似葡萄糖氧化酶的能力。我们提出的镍纳米酶对葡萄糖表现出683 μA mM cm的良好灵敏度。该传感器在0.001-3.1 mM范围内工作，检测下限（LOD）为9.1 μM，响应时间约为0.1 s。镍纳米酶在存在干扰化合物的情况下对葡萄糖表现出更好的选择性。值得注意的是，该传感器不会受到析氧反应的干扰。这大大提高了低浓度葡萄糖检测的灵敏度，使该传感器能够用于测量唾液葡萄糖水平。在本研究中，我们证明了我们的传感器能够检测人类唾液中的葡萄糖。对三名健康人类志愿者和一名糖尿病前期志愿者的唾液样本进行了实际样品分析。我们提出的传感器测量结果与计算出的唾液葡萄糖水平显示出极好的一致性，在实际唾液样本中检测葡萄糖的准确率为98%。
