Agroécologie, InstitutAgro Dijon, INRAE, Université de Bourgogne, Université de Bourgogne Franche-Comté, Dijon, France.
Methods Mol Biol. 2024;2827:109-143. doi: 10.1007/978-1-0716-3954-2_8.
Plant growth regulators are routinely added to in vitro culture media to foster the growth and differentiation of the cells, tissues, and organs. However, while the literature on usage of the more common auxins, cytokinins, gibberellins, abscisic acid, and ethylene is vast, other compounds that also have shown a growth-regulating activity have not been studied as frequently. Such substances are also capable of modulating the responses of plant cells and tissues in vitro by regulating their growth, differentiation, and regeneration competence, but also by enhancing their responses toward biotic and abiotic stress agents and improving the production of secondary metabolites of interest. This chapter will discuss the in vitro effects of several of such less frequently added plant growth regulators, including brassinosteroids (BRS), strigolactones (SLs), phytosulfokines (PSKs), methyl jasmonate, salicylic acid (SA), sodium nitroprusside (SNP), hydrogen sulfite, various plant growth retardants and inhibitors (e.g., ancymidol, uniconazole, flurprimidol, paclobutrazol), and polyamines.
植物生长调节剂通常被添加到体外培养基中，以促进细胞、组织和器官的生长和分化。然而，虽然关于更常见的生长素、细胞分裂素、赤霉素、脱落酸和乙烯的使用的文献很多，但其他也表现出生长调节活性的化合物并没有被频繁研究。这些物质也能够通过调节植物细胞和组织的生长、分化和再生能力来调节其对生物和非生物胁迫剂的反应，同时还可以提高感兴趣的次生代谢产物的产量。本章将讨论几种添加频率较低的植物生长调节剂的体外效应，包括油菜素内酯（BRS）、独脚金内酯（SLs）、根皮素（PSKs）、茉莉酸甲酯、水杨酸（SA）、硝普钠（SNP）、亚硫酸氢盐、各种植物生长延缓剂和抑制剂（如安克菌、烯效唑、氟啶菌胺、多效唑）和多胺。
