Posada-Perez Laisyn, Gómez-Kosky Rafael, Padron Yenny, Galvez Diosdada, Daniels Dion
Instituto de Biotecnología de las plantas (IBP), Universidad Central "Marta Abreu" de Las Villas, Santa Clara, Villa Clara, Cuba.
Estación Territorial de Investigaciones de la caña de azúcar (ETICA Centro Villa Clara), Instituto de Investigaciones de la caña de azúcar (INICA), Ranchuelo, Villa Clara, Cuba.
Methods Mol Biol. 2022;2527:143-157. doi: 10.1007/978-1-0716-2485-2_12.
High mortality rates of in vitro plants during ex vitro acclimatization, due to low rooting, is one of the main problems of papaya tissue culture. This work was carried out with the objective to obtain 100% hermaphroditic in vitro plants of the papaya cultivar "Maradol Roja" by somatic embryogenesis, which have an adequate rooting system that allows them a higher survival percentage in the ex vitro acclimatization phase. In international scientific literature, there are several protocols; however, not all of them cover the different phases of somatic embryogenesis. This chapter describes a complete and optimized protocol from immature zygotic embryos in this cultivar. It also looks at the morpho-anatomical characterization of somatic embryos in the different stages of ontogenetic development, as well as high survival rates under ex vitro conditions of the plants obtained. It can be used for genetic improvement and propagation of this species.
由于生根率低，离体植株在驯化期间死亡率很高，这是木瓜组织培养的主要问题之一。本研究旨在通过体细胞胚胎发生获得100%的“马拉多尔·罗哈”木瓜品种雌雄同株离体植株，这些植株具有适宜的根系，使其在驯化阶段具有更高的成活率。在国际科学文献中有几种方案；然而，并非所有方案都涵盖体细胞胚胎发生的不同阶段。本章描述了从该品种未成熟合子胚开始的完整且优化的方案。还研究了体细胞胚胎在个体发育不同阶段的形态解剖特征，以及所获植株在离体条件下的高成活率。它可用于该物种的遗传改良和繁殖。
