Department of Biology, College of Science, Sultan Qaboos University, Muscat, Oman.
Methods Mol Biol. 2024;2827:385-404. doi: 10.1007/978-1-0716-3954-2_26.
Abiotic environmental stressors cause various types of damage to plants and cause significant loss in yield. Abiotic stress tolerance in plants refers to the ability to withstand environmental factors and maintain growth, development, and production. Since this tolerance is controlled by a gene or a set of genes, transgenic activating of these genes in plants often enhances tolerance under abiotic stress. Therefore, this methodology chapter describes a strategy and the corresponding protocols needed to induce a gene by an abiotic stressor, clone the corresponding cDNA into plasmids and Agrobacterium cells, and genetic transformation to the Arabidopsis plants using the floral dip method. The chapter also describes standard assays to evaluate the transgene's effect on the plant's tolerance. Finally, the techniques outlined in this chapter for cloning and generating transgenic plants tolerant to abiotic stress are a versatile approach that can be implemented across various plant species and genes.
非生物环境胁迫会对植物造成各种类型的损伤，并导致产量的重大损失。植物的非生物胁迫耐受能力是指其承受环境因素并维持生长、发育和生产的能力。由于这种耐受能力受一个或一组基因控制，因此在植物中过表达这些基因通常会增强其在非生物胁迫下的耐受能力。因此，本方法学章节描述了一种策略和相应的方案，通过非生物胁迫诱导基因，将相应的 cDNA 克隆到质粒和农杆菌细胞中，并使用花浸法进行遗传转化到拟南芥植物中。该章节还描述了评估转基因对植物耐受能力影响的标准检测方法。最后，本章中概述的用于克隆和生成耐受非生物胁迫的转基因植物的技术是一种通用方法，可以应用于各种植物物种和基因。
