[破伤风溶血素制剂的制备及其性质特点]
[Production of tetanolysin preparations and characteristics of their properties].
作者信息
Shabarov I A, Rumiantsev S N, Pospelov V F
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 1985 Jun(6):3-6.
As the result of the study of tetanolysin-producing Clostridium tetani strains, their populations have been found to be markedly heterogeneous with respect to the hemolytic activity of clone cultures. On the basis of normal and dialyzed cultures of selected variants with maximum activity the preparations of tetanolysin have been obtained, and their hemolytic activity and antigenic properties have been studied. Antihemolytic rabbit sera have also been obtained and characterized. Partially purified preparations of tetanolysin with high hemolytic activity have been obtained by the fractionation of C. tetani dialyzed cultures with ammonium sulfate.
作为对产破伤风溶血素破伤风梭菌菌株研究的结果，已发现它们的群体在克隆培养物的溶血活性方面存在明显的异质性。基于具有最大活性的选定变体的正常培养物和透析培养物，获得了破伤风溶血素制剂，并研究了它们的溶血活性和抗原特性。还获得并表征了抗溶血兔血清。通过用硫酸铵对破伤风梭菌透析培养物进行分级分离，获得了具有高溶血活性的部分纯化的破伤风溶血素制剂。
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