Tanaka N, Christensen P, Rydén S, Klöfver-Ståhl B, Bengmark S
Acta Pathol Microbiol Immunol Scand B. 1985 Jun;93(3):171-4. doi: 10.1111/j.1699-0463.1985.tb02872.x.
The effect of retrograde intrabiliary (RI) injection of E. coli was studied in Sprague-Dawley rats with and without chronic biliary obstruction. The challenge dose of E. coli was standardized by the use of frozen (-80 degrees C) aliquots of bacteria. Injection of 10(2), 10(5) or 10(8) colony-forming units (CFU), respectively, into three groups of 8 normal rats, immediately after occlusion of the common bile duct (CBD), did not kill any of the animals. In contrast, 5 of 8 animals with chronic biliary obstruction died from E. coli sepsis after RI injection of 10(2) bacteria (p less than 0.05). Furthermore, all of 4 obstructed animals died after challenge with 10(5) CFU (p less than 0.01), as compared to the 8 normal rats surviving this dose. Intraperitoneal injection of 10(5) E. coli did not kill any of 6 animals with 3 weeks biliary obstruction. It is concluded that chronic biliary obstruction and RI injection are prerequisites for the occurrence of lethal septicemia in the animals. The model might be suitable for the study of biliary sepsis in chronic biliary obstruction.
在有或没有慢性胆管梗阻的Sprague-Dawley大鼠中，研究了逆行胆管内（RI）注射大肠杆菌的效果。大肠杆菌的激发剂量通过使用冷冻（-80摄氏度）的细菌等分试样进行标准化。在胆总管（CBD）闭塞后，立即分别向三组8只正常大鼠注射10²、10⁵或10⁸菌落形成单位（CFU），没有一只动物死亡。相比之下，8只患有慢性胆管梗阻的动物中有5只在RI注射10²个细菌后死于大肠杆菌败血症（p<0.05）。此外，与8只在该剂量下存活的正常大鼠相比，4只梗阻动物在接受10⁵CFU攻击后全部死亡（p<0.01）。对6只患有3周胆管梗阻的动物进行腹腔注射10⁵个大肠杆菌，没有一只死亡。结论是慢性胆管梗阻和RI注射是动物发生致命败血症的先决条件。该模型可能适用于慢性胆管梗阻中胆源性败血症的研究。
