Division of Environmental Chemistry, National Institute of Health Sciences.
Department of Allergology, Fujita Health University School of Medicine.
Chem Pharm Bull (Tokyo). 2024;72(7):664-668. doi: 10.1248/cpb.c24-00278.
Henna is a plant-based dye obtained from the powdered leaf of the pigmented plant Lawsonia inermis, and has often been used for grey hair dyeing, treatment, and body painting. As a henna product, the leaves of Indigofera tinctoria and Cassia auriculata can be blended to produce different colour variations. Although allergy from henna products attributed to p-phenylenediamine, which is added to enhance the dye, is reported occasionally, raw material plants of henna products could also contribute to the allergy. In this study, we reported that raw material plants of commercial henna products distributed in Japan can be estimated by LC-high resolution MS (LC-HRMS) and multivariate analysis. Principal Component Analysis (PCA) score plot clearly separated 17 samples into three groups [I; henna, II; blended henna primarily comprising Indigofera tinctoria, III; Cassia auriculata]. This grouping was consistent with the ingredient lists of products except that one sample listed as henna was classified as Group III, indicating that its ingredient label may differ from the actual formulation. The ingredients characteristic to Groups I, II, and III by PCA were lawsone (1), indirubin (2), and rutin (3), respectively, which were reported to be contained in each plant as ingredients. Therefore, henna products can be considered to have been manufactured from these plants. This study is the first to estimate raw material plants used in commercial plant-based dye by LC-HRMS and multivariate analysis.
指甲花是一种植物染料，从染色植物散沫花的粉末状叶子中提取而成，常用于灰色头发染发、治疗和身体彩绘。作为指甲花产品，菘蓝和小槐花的叶子可以混合产生不同的颜色变化。尽管偶尔会报道由于添加到增强染料中的对苯二胺引起的指甲花产品过敏，但指甲花产品的原料植物也可能导致过敏。在这项研究中，我们报告了通过 LC-高分辨率 MS（LC-HRMS）和多元分析可以估计在日本销售的商业指甲花产品的原料植物。主成分分析（PCA）得分图清楚地将 17 个样品分为三组 [I；指甲花，II；主要由菘蓝组成的混合指甲花，III；小槐花]。这种分组与产品的成分清单一致，除了一个列为指甲花的样品被归类为第 III 组外，这表明其成分标签可能与实际配方不同。通过 PCA 确定的组 I、II 和 III 的特征成分分别为 1 号 lawsone、2 号 indirubin 和 3 号芦丁，这些成分据报道分别存在于每种植物中作为成分。因此，可以认为指甲花产品是由这些植物制成的。本研究首次通过 LC-HRMS 和多元分析来估计商业植物性染料中使用的原料植物。
