Wales Thomas E, Pajak Aleksandra, Roeselová Alžběta, Shivakumaraswamy Santosh, Howell Steven, Kjær Svend, Hartl F Ulrich, Engen John R, Balchin David
Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA.
Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK.
Nat Struct Mol Biol. 2024 Dec;31(12):1888-1897. doi: 10.1038/s41594-024-01355-x. Epub 2024 Jul 10.
Protein folding in vivo begins during synthesis on the ribosome and is modulated by molecular chaperones that engage the nascent polypeptide. How these features of protein biogenesis influence the maturation pathway of nascent proteins is incompletely understood. Here, we use hydrogen-deuterium exchange mass spectrometry to define, at peptide resolution, the cotranslational chaperone-assisted folding pathway of Escherichia coli dihydrofolate reductase. The nascent polypeptide folds along an unanticipated pathway through structured intermediates not populated during refolding from denaturant. Association with the ribosome allows these intermediates to form, as otherwise destabilizing carboxy-terminal sequences remain confined in the ribosome exit tunnel. Trigger factor binds partially folded states without disrupting their structure, and the nascent chain is poised to complete folding immediately upon emergence of the C terminus from the exit tunnel. By mapping interactions between the nascent chain and ribosomal proteins, we trace the path of the emerging polypeptide during synthesis. Our work reveals new mechanisms by which cellular factors shape the conformational search for the native state.
体内蛋白质折叠始于核糖体上的合成过程，并受到与新生多肽结合的分子伴侣的调节。蛋白质生物合成的这些特征如何影响新生蛋白质的成熟途径，目前尚不完全清楚。在这里，我们使用氢-氘交换质谱法，在肽段分辨率水平上确定大肠杆菌二氢叶酸还原酶的共翻译伴侣辅助折叠途径。新生多肽沿着一条意想不到的途径折叠，通过在从变性剂中重折叠过程中未出现的结构化中间体。与核糖体的结合使这些中间体得以形成，因为原本会使结构不稳定的羧基末端序列仍被限制在核糖体出口通道中。触发因子结合部分折叠状态而不破坏其结构，并且新生链在C末端从出口通道出现后立即准备完成折叠。通过绘制新生链与核糖体蛋白之间的相互作用，我们追踪了合成过程中新生多肽的路径。我们的工作揭示了细胞因子塑造天然状态构象搜索的新机制。
