从含有干扰化合物的血浆中制备、提取环孢素A并进行高效液相色谱分析。

Preparation, extraction and high performance liquid chromatography of cyclosporin A from plasma that contains interfering compounds.

作者信息

Oosthuizen M M, Jacobs D R, Myburgh J A

出版信息

Ann Clin Biochem. 1985 Jul;22 ( Pt 4):402-7. doi: 10.1177/000456328502200413.

DOI:10.1177/000456328502200413

Abstract

Cyclosporin A (CyA) in human plasma-containing interfering compounds was determined by high performance liquid chromatography (HPLC) after the plasma had been subjected to incubation with proteinase K. This hydrolytic enzyme did not hydrolyse the cyclosporins and was therefore an ideal aid in eliminating interfering peptides selectively. The use of Extrelut as an extraction aid contributed much to higher yields of cyclosporins. Ethyl acetate, the extraction solvent used, gave less complex profiles on HPLC chromatograms than other solvents (eg diethyl ether and methanol) and contributed to a shorter chromatographic period per sample, an advantage in repetitive determinations.

摘要

含干扰化合物的人血浆中的环孢素A（CyA），在血浆与蛋白酶K孵育后，通过高效液相色谱法（HPLC）进行测定。这种水解酶不会水解环孢菌素，因此是选择性消除干扰肽的理想辅助手段。使用埃特拉吕特（Extrelut）作为萃取辅助剂，对提高环孢菌素的产率有很大帮助。所使用的萃取溶剂乙酸乙酯，在HPLC色谱图上给出的图谱比其他溶剂（如乙醚和甲醇）更简单，并且有助于缩短每个样品的色谱分析时间，这在重复测定中是一个优势。