一种用于全血和血浆中环孢素A的高效液相色谱法的改进提取程序。
An improved extraction procedure for an HPLC method for cyclosporin A in whole blood and plasma.
作者信息
Maguire S, Kyne F, UaConaill D
出版信息
Ann Clin Biochem. 1987 Mar;24 ( Pt 2):161-6. doi: 10.1177/000456328702400206.
PMID:3592565
Abstract
Cyclosporin A (CyA) was assayed by high performance liquid chromatography (HPLC) following extraction of the whole blood or plasma with diethyl ether and chromatography on a Sep-Pak Silica cartridge. Using carefully chosen volumes and ratios of ethyl acetate and hexane in the eluting sequences on the Sep-Pak, the major interferent present in normal blood and serum was eliminated.
摘要
采用高效液相色谱法（HPLC）测定环孢素A（CyA），方法是先用乙醚提取全血或血浆，然后在Sep-Pak硅胶柱上进行色谱分析。通过在Sep-Pak柱洗脱过程中精心选择乙酸乙酯和己烷的体积及比例，消除了正常血液和血清中存在的主要干扰物。
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