Fletcher S J, Bacchus R A
Department of Pathology, Riyadh Armed Forces Hospital, Kingdom of Saudi Arabia.
Ann Clin Biochem. 1988 Sep;25 ( Pt 5):510-5. doi: 10.1177/000456328802500506.
The current HPLC methods of cyclosporin measurement have been reviewed and all aspects assessed. A simple isocratic C-18 reverse phase HPLC method with improved efficiency is described for the routine measurement of cyclosporin in whole blood. An alkaline ether extraction is followed by an acid wash, solvent evaporation and two hexane washes of the reconstituted extract. The turn-round time for a single sample is 1 h. Daily batches of up to 40 patient samples can be easily measured with this method. The results are compared with those from the Sandoz radioimmunoassay (RIA) method.
对目前用于测量环孢素的高效液相色谱法进行了综述，并对各个方面进行了评估。描述了一种简单的等度C-18反相高效液相色谱法，该方法提高了效率，用于全血中环孢素的常规测量。采用碱性乙醚萃取，随后进行酸洗、溶剂蒸发以及对复溶提取物进行两次己烷洗涤。单个样品的周转时间为1小时。使用该方法可以轻松测量每日批次多达40个患者样本。将结果与山德士放射免疫测定（RIA）法的结果进行比较。
