Department of Ecology and Evolutionary Biology, University of Connecticut, 75 North Eagleville Road, Storrs, CT 06269-3043, USA.
Department of Ecology and Evolutionary Biology, University of Connecticut, 75 North Eagleville Road, Storrs, CT 06269-3043, USA.
Cell Rep. 2024 Jul 23;43(7):114444. doi: 10.1016/j.celrep.2024.114444. Epub 2024 Jul 10.
The emergence of novel traits is often preceded by a potentiation phase, when all the genetic components necessary for producing the trait are assembled. However, elucidating these potentiating factors is challenging. We have previously shown that an anthocyanin-activating R2R3-MYB, STRIPY, triggers the emergence of a distinct foliar pigmentation pattern in the monkeyflower Mimulus verbenaceus. Here, using forward and reverse genetics approaches, we identify three potentiating factors that pattern STRIPY expression: MvHY5, a master regulator of light signaling that activates STRIPY and is expressed throughout the leaf, and two leaf developmental regulators, MvALOG1 and MvTCP5, that are expressed in opposing gradients along the leaf proximodistal axis and negatively regulate STRIPY. These results provide strong empirical evidence that phenotypic novelties can be potentiated through incorporation into preexisting genetic regulatory networks and highlight the importance of positional information in patterning the novel foliar stripe.
新特征的出现通常先经历一个增强阶段，在此阶段，产生该特征所需的所有遗传成分都被组装在一起。然而，阐明这些增强因子具有挑战性。我们之前曾表明，花青素激活的 R2R3-MYB 蛋白 STRIPY 触发猴面花 Mimulus verbenaceus 中独特的叶片色素沉着模式的出现。在这里，我们使用正向和反向遗传学方法，鉴定了三个增强 STRIPY 表达的增强因子：MvHY5，它是光信号的主要调节因子，激活 STRIPY 并在整个叶片中表达；以及两个叶片发育调节剂 MvALOG1 和 MvTCP5，它们沿着叶片近-远轴以相反的梯度表达，并负调控 STRIPY。这些结果提供了强有力的经验证据，表明表型新颖性可以通过纳入预先存在的遗传调控网络来增强，并强调了位置信息在模式化新叶片条纹中的重要性。
