Tao Chuming, Liu Chenglong, Ge Peicong, Chan Liujia, Pang Yuheng, Li Junsheng, He Qiheng, Liu Wei, Mou Siqi, Zheng Zhiyao, Zhang Bojian, Zhao Zhikang, Sun Wei, Zhang Qian, Wang Rong, Zhang Yan, Wang Wenjing, Zhang Dong, Zhao Jizong
Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, China.
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Clin Exp Immunol. 2025 Jan 21;219(1). doi: 10.1093/cei/uxae058.
Growing evidence suggests that systemic immune and inflammatory responses may play a critical role in the formation and development of aneurysms. Exploring the differences between single intracranial aneurysm (SIA) and multiple IAs (MIAs) could provide insights for targeted therapies. However, there is a lack of comprehensive and detailed characterization of changes in circulating immune cells in MIAs. Peripheral blood mononuclear cell (PBMC) samples from patients with SIA (n = 16) or MIAs (n = 6) were analyzed using high-dimensional mass cytometry to evaluate the frequency and phenotype of immune cell subtypes. A total of 25 cell clusters were identified, revealing that the immune signature of MIAs included cluster changes. Compared to patients with SIA, patients with MIAs exhibited immune dysfunction and regulatory imbalance in T-cell clusters. They also had reduced numbers of CD8+ T cells and their subgroups CD8+ Te and CD8+ Tem cells, as well as reduced numbers of the CD4+ T-cell subgroup CD27-CD4+ Tem cells. Furthermore, compared to SIA, MIAs were associated with enhanced T-cell immune activation, with elevated expression levels of CD3, CD25, CD27, CCR7, GP130, and interleukin 10. This study provides insights into the circulating immune cell profiles in patients with MIAs, highlighting the similarities and differences between patients with SIA and those with MIAs. Furthermore, the study suggests that circulating immune dysfunction may contribute to the development of MIAs.
越来越多的证据表明，全身免疫和炎症反应可能在动脉瘤的形成和发展中起关键作用。探索单发颅内动脉瘤（SIA）和多发颅内动脉瘤（MIA）之间的差异可为靶向治疗提供思路。然而，目前缺乏对MIA患者循环免疫细胞变化的全面而详细的特征描述。使用高维质谱流式细胞术分析了SIA患者（n = 16）或MIA患者（n = 6）的外周血单个核细胞（PBMC）样本，以评估免疫细胞亚群的频率和表型。共鉴定出25个细胞簇，揭示MIA的免疫特征包括簇变化。与SIA患者相比，MIA患者在T细胞簇中表现出免疫功能障碍和调节失衡。他们的CD8 + T细胞及其亚群CD8 + Te和CD8 + Tem细胞数量也减少，以及CD4 + T细胞亚群CD27-CD4 + Tem细胞数量减少。此外，与SIA相比，MIA与T细胞免疫激活增强有关，CD3、CD25、CD27、CCR7、GP130和白细胞介素10的表达水平升高。本研究为MIA患者的循环免疫细胞谱提供了见解，突出了SIA患者和MIA患者之间的异同。此外，该研究表明循环免疫功能障碍可能促成MIA的发生。
