酵母中产生的乙型肝炎表面抗原的特性

Characteristics of hepatitis B surface antigen produced in yeast.

作者信息

Choo K B, Wu S M, Lee H H, Lo S C

出版信息

Biochem Biophys Res Commun. 1985 Aug 30;131(1):160-6. doi: 10.1016/0006-291x(85)91784-x.

Abstract

We have constructed an expression plasmid for regulated expression of the hepatitis B surface antigen gene in yeast using promoter of the yeast Pho5 gene. In the yeast transformants, the monomeric HBsAg (22K dalton) was estimated to constitute approximately 3% of the total proteins. On extraction, the HBsAg was found to have a buoyant density of 1.18 g/ml and an Sw.20 value of 54. Electron microscopy revealed particles of heterogeneous size ranging from 18-28 nm. When the yeast HBsAg was used to immunize guinea pigs, the anti-HBsAg antibodies produced could react with human serum HBsAg.

摘要

我们利用酵母Pho5基因的启动子构建了一种表达质粒，用于在酵母中调控乙型肝炎表面抗原基因的表达。在酵母转化体中，单体HBsAg（22K道尔顿）估计约占总蛋白的3%。提取后发现，HBsAg的浮力密度为1.18 g/ml，沉降系数Sw.20值为54。电子显微镜显示颗粒大小不一，范围为18 - 28纳米。当用酵母HBsAg免疫豚鼠时，产生的抗HBsAg抗体可与人血清HBsAg发生反应。