RECA免疫分析作为一种分析SOS反应的工具。

RECA immunological assay as a tool to analyze the SOS response.

作者信息

Salles B, Calsou P, Defais M

出版信息

Biochimie. 1985 Mar-Apr;67(3-4):349-52. doi: 10.1016/s0300-9084(85)80078-x.

Abstract

The content of RECA protein, one of the SOS genes product, was determined in a bacterial extract by a two site-radioimmunometric assay. The variation of the RECA concentration after induction by physical or chemical treatments was used as a probe to analyze the SOS response. Relationships between either the number or the nature of DNA lesions and the level of the relative amplification of RECA have been established. The modulation of the recA gene expression is discussed.

摘要

通过双位点放射免疫测定法测定了细菌提取物中SOS基因产物之一的RECA蛋白含量。将物理或化学处理诱导后RECA浓度的变化用作分析SOS反应的探针。已经建立了DNA损伤的数量或性质与RECA相对扩增水平之间的关系。并对recA基因表达的调节进行了讨论。