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通过RecA蛋白扩增分析SOS反应的调控。

Regulation of the SOS response analyzed by RecA protein amplification.

作者信息

Calsou P, Salles B

出版信息

J Bacteriol. 1985 Jun;162(3):1162-5. doi: 10.1128/jb.162.3.1162-1165.1985.

DOI:10.1128/jb.162.3.1162-1165.1985
PMID:3888960
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215898/
Abstract

A split UV light dose procedure was used in Escherichia coli to induce an SOS function, RecA protein amplification, which was measured by an immunoradiometric assay. The SOS system was partially induced after the first UV irradiation, and the inducing effects of subsequent identical UV doses were quantified. Variations in the inducing effects of successive UV doses were related to modulations of the SOS signal level during SOS induction. A reduction in the level of SOS signal was found after 20 min in the wild-type strain, hypothesized to result from negative control of repair functions. A few DNA repair mutants were tested by the same procedure; the uvrA, recF, and umuC genes were involved in SOS induction control, but we found differences in their respective kinetics of expression. On the contrary, in a recB mutant, only a slight effect was obtained on this control.

摘要

采用分裂紫外线剂量程序在大肠杆菌中诱导SOS功能、RecA蛋白扩增，通过免疫放射分析进行测量。首次紫外线照射后SOS系统被部分诱导，后续相同紫外线剂量的诱导效果被量化。连续紫外线剂量诱导效果的变化与SOS诱导过程中SOS信号水平的调节有关。在野生型菌株中，20分钟后发现SOS信号水平降低，推测这是由修复功能的负调控导致的。通过相同程序测试了一些DNA修复突变体；uvrA、recF和umuC基因参与SOS诱导控制，但我们发现它们各自的表达动力学存在差异。相反，在recB突变体中，此控制仅获得轻微效果。

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本文引用的文献

1
The SOS regulatory system of Escherichia coli.大肠杆菌的SOS调控系统。
Cell. 1982 May;29(1):11-22. doi: 10.1016/0092-8674(82)90085-x.
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An immunoradiometric quantitative assay of Escherichia coli recA protein.
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Inducibility of a gene product required for UV and chemical mutagenesis in Escherichia coli.大肠杆菌中紫外线和化学诱变所需基因产物的可诱导性。
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The lexA gene product represses its own promoter.lexA基因产物可抑制其自身的启动子。
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Microbiol Rev. 1984 Mar;48(1):60-93. doi: 10.1128/mr.48.1.60-93.1984.
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Role of the E. coli umuC gene product in the repair of single-stranded DNA phage.大肠杆菌umuC基因产物在单链DNA噬菌体修复中的作用。
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8
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