Institute of Medicinal Plant Cultivation, Academy of Southern Medicine, College of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China.
Yunnan Normal University, Kunming, 650500, China.
Sci Rep. 2024 Jul 11;14(1):16061. doi: 10.1038/s41598-024-67040-7.
Rhizome rot is a destructive soil-borne disease of Polygonatum kingianum and adversely affects the yield and sustenance of the plant. Understanding how the causal fungus Fusarium oxysporum infects P. kingianum may suggest effective control measures against rhizome rot. In germinating conidia of infectious F. oxysporum, expression of the zinc finger transcription factor gene Zfp1, consisting of two CH motifs, was up-regulated. To characterize the critical role of ZFP1, we generated independent deletion mutants (zfp1) and complemented one mutant with a transgenic copy of ZFP1 (zfp1 tZFP1). Mycelial growth and conidial production of zfp1 were slower than those of wild type (ZFP1) and zfp1 tZFP1. Additionally, a reduced inhibition of growth suggested zfp1 was less sensitive to conditions promoting cell wall and osmotic stresses than ZFP1 and zfp1 tZFP1. Furthermore pathogenicity tests suggested a critical role for growth of zfp1 in infected leaves and rhizomes of P. kingianum. Thus ZFP1 is important for mycelial growth, conidiation, osmoregulation, and pathogenicity in P. kingianum.
块根腐烂病是一种破坏百合科黄精属植物的土传病害，会对植物的产量和生存造成不利影响。了解致病真菌尖孢镰刀菌如何感染黄精可能会提出针对块根腐烂病的有效防治措施。在具有感染力的尖孢镰刀菌的萌发分生孢子中，由两个 CH 基序组成的锌指转录因子基因 Zfp1 的表达被上调。为了描述 ZFP1 的关键作用，我们生成了独立的缺失突变体（zfp1），并通过转基因拷贝（zfp1 tZFP1）对一个突变体进行了互补。zfp1 的菌丝生长和分生孢子产生速度比野生型（ZFP1）和 zfp1 tZFP1 慢。此外，抑制生长的能力降低表明，与 ZFP1 和 zfp1 tZFP1 相比，zfp1 对促进细胞壁和渗透胁迫的条件的敏感性较低。此外，致病性测试表明 zfp1 在感染的黄精叶片和块茎中的生长起关键作用。因此，ZFP1 对于黄精的菌丝生长、分生孢子形成、渗透压调节和致病性非常重要。
