State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
Fujian Institute for Food and Drug Quality Control, Fuzhou, China.
Mol Plant Pathol. 2021 Jul;22(7):882-895. doi: 10.1111/mpp.13068. Epub 2021 May 9.
Fusarium oxysporum is a well-known soilborne plant pathogen that causes severe vascular wilt in economically important crops worldwide. During the infection process, F. oxysporum not only secretes various virulence factors, such as cell wall-degrading enzymes (CWDEs), effectors, and mycotoxins, that potentially play important roles in fungal pathogenicity but it must also respond to extrinsic abiotic stresses from the environment and the host. Over 700 transcription factors (TFs) have been predicted in the genome of F. oxysporum, but only 26 TFs have been functionally characterized in various formae speciales of F. oxysporum. Among these TFs, a total of 23 belonging to 10 families are required for pathogenesis through various mechanisms and pathways, and the zinc finger TF family is the largest family among these 10 families, which consists of 15 TFs that have been functionally characterized in F. oxysporum. In this review, we report current research progress on the 26 functionally analysed TFs in F. oxysporum and sort them into four groups based on their roles in F. oxysporum pathogenicity. Furthermore, we summarize and compare the biofunctions, involved pathways, putative targets, and homologs of these TFs and analyse the relationships among them. This review provides a systematic analysis of the regulation of virulence-related genes and facilitates further mechanistic analysis of TFs important in F. oxysporum virulence.
尖孢镰刀菌是一种众所周知的土壤传播植物病原体，它会导致全球经济重要作物的严重维管束萎蔫。在感染过程中，尖孢镰刀菌不仅会分泌各种毒力因子，如细胞壁降解酶（CWDEs）、效应子和真菌毒素，这些因子可能在真菌致病性中发挥重要作用，还必须应对环境和宿主的外在非生物胁迫。在尖孢镰刀菌的基因组中预测了超过 700 个转录因子（TFs），但仅在尖孢镰刀菌的各种特殊形式中对 26 个 TFs 进行了功能表征。在这些 TFs 中，总共有 23 个属于 10 个家族的 TFs 通过各种机制和途径参与致病，锌指 TF 家族是这 10 个家族中最大的家族，其中包含 15 个在尖孢镰刀菌中功能表征的 TFs。在这篇综述中，我们报告了尖孢镰刀菌中 26 个功能分析 TFs 的最新研究进展，并根据它们在尖孢镰刀菌致病性中的作用将它们分为四组。此外，我们总结和比较了这些 TFs 的生物功能、涉及的途径、假定的靶标和同源物，并分析了它们之间的关系。这篇综述提供了对与毒力相关基因的调控的系统分析，并促进了对尖孢镰刀菌毒力中重要 TFs 的进一步机制分析。
