Chen Yiwen, Lin Ying, Zhang Yuanhui, Liu Xiaoping, Jiang Ming
Department of Otolaryngology Head and Neck Surgery, Guangzhou Red Cross Hospital (Guangzhou Red Cross Hospital of Jinan University), Guangzhou, Guangdong, 510240, China.
Department of Otolaryngology Head and Neck Surgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China.
Heliyon. 2024 Jun 13;10(12):e32952. doi: 10.1016/j.heliyon.2024.e32952. eCollection 2024 Jun 30.
Sensorineural hearing loss (SNHL) is a prevalent condition in otolaryngology. A key obstacle is finding effective strategies for regenerating damaged cochlear hair cells in adult animals. A practical and reliable approach has been developed to create a superior cell source for stem cell transplantation in the inner ear to treat SNHL. is involved in the differentiation of neurons, intestinal secretory cells, and mechanoreceptors including auditory hair cells, and thus plays an important role in neurogenesis. Lentivirus-mediated transfection of bone marrow mesenchymal stem cells (BMSCs) was utilized to achieve stable expression of the essential transcription factor , which is crucial for developing auditory hair cells without compromising cell survival. By manipulating the induction conditions through altering the cell growth environment using anti-adherent culture, the synergistic impact of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) was effectively applied to significantly improve the differentiation efficiency of bone marrow-derived mesenchymal stem cells (BMSC) into neural stem cells (NSCs) following transfection, thereby reducing the induction time. The study indicated that the newly proposed transdifferentiation method effectively transformed BMSCs into NSCs in a controlled environment, presenting a potential approach for stem cell transplantation to promote hair cell regeneration.
感音神经性听力损失（SNHL）是耳鼻喉科的一种常见病症。一个关键障碍是找到在成年动物中再生受损耳蜗毛细胞的有效策略。已经开发出一种实用且可靠的方法，以创建一种优质的细胞来源用于内耳干细胞移植来治疗SNHL。 参与神经元、肠道分泌细胞以及包括听觉毛细胞在内的机械感受器的分化，因此在神经发生中起重要作用。利用慢病毒介导的骨髓间充质干细胞（BMSC）转染来实现关键转录因子的稳定表达，这对于发育听觉毛细胞至关重要且不影响细胞存活。通过使用抗黏附培养改变细胞生长环境来操纵诱导条件，有效应用碱性成纤维细胞生长因子（bFGF）和表皮生长因子（EGF）的协同作用，显著提高转染后骨髓间充质干细胞（BMSC）向神经干细胞（NSC）的分化效率，从而缩短诱导时间。该研究表明，新提出的转分化方法在可控环境中有效地将BMSC转化为NSC，为促进毛细胞再生的干细胞移植提供了一种潜在方法。
