SDU-ANU Joint Science College, Shandong University, Weihai, Shandong, 264209, PR China.
Marine College, Shandong University, Weihai, Shandong, 264209, PR China.
Int J Syst Evol Microbiol. 2024 Jul;74(7). doi: 10.1099/ijsem.0.006448.
A novel facultatively anaerobic and Gram-stain-negative bacterium, designated FJH33, was isolated from mangrove sediment sampled in Zhangzhou, PR China. Cells of strain FJH33 were rod-shaped or slightly curved-shaped, with widths of 0.3-0.5 µm and lengths of 1.0-3.0 µm. Optimum growth of strain FJH33 occurred in the presence of 3 % NaCl (w/v), at 33 °C and at pH 7.0. Oxidase activity was negative, while catalase activity was positive. Its iron-reducing ability was determined. Based on 16S rRNA gene sequence similarity, strain FJH33 was most closely related to XSD2 (95.1 %), followed by Y2-1-60 (95.0 %) and 5E3 (94.9 %). Genome analysis of strains FJH33 and XSD2 revealed low genome relatedness, with an average nucleotide identity value of 73.8% and a digital DNA-DNA hybridization value of 19.0%. Phylogenetic trees built from 16S rRNA genes and genome sequences showed that strain FJH33 represents a relatively independent phylogenetic lineage within the genus . The major cellular fatty acids (≥10 %) were iso-C and C 9. The sole respiratory quinone was MK-7. The polar lipids consisted of phosphatidylethanolamine, diphosphatidylcholine, diphosphatidyglycerol and one unidentified lipid. The DNA G+C content was 41.4 mol%. Based on the integrated results of phylogenetic, physiological, biochemical and chemotaxonomic characterizations, we propose that strain FJH33 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FJH33 (=KCTC 102210=MCCC 1H01459).
一株新的兼性厌氧革兰氏阴性细菌，被命名为 FJH33，从中国福建漳州红树林沉积物中分离得到。FJH33 菌株的细胞呈杆状或略弯曲状，宽度为 0.3-0.5μm，长度为 1.0-3.0μm。FJH33 菌株的最佳生长条件为 3%NaCl(w/v)、33°C 和 pH7.0。氧化酶活性为阴性，而过氧化氢酶活性为阳性。该菌具有铁还原能力。基于 16S rRNA 基因序列相似性，FJH33 菌株与 XSD2（95.1%）最为密切相关，其次是 Y2-1-60（95.0%）和 5E3（94.9%）。FJH33 株和 XSD2 株的基因组分析表明，它们的基因组相关性较低，平均核苷酸同一性值为 73.8%，数字 DNA-DNA 杂交值为 19.0%。基于 16S rRNA 基因和基因组序列构建的系统发育树表明，FJH33 株代表了属内一个相对独立的系统发育分支。主要的细胞脂肪酸（≥10%）为 iso-C 和 C9。唯一的呼吸醌为 MK-7。极性脂由磷脂酰乙醇胺、双磷脂酰胆碱、双磷脂酰甘油和一种未鉴定的脂组成。DNA G+C 含量为 41.4mol%。基于系统发育、生理、生化和化学分类特征的综合结果，我们建议 FJH33 株代表一个新的属种，命名为 sp. nov.。模式菌株为 FJH33（=KCTC 102210=MCCC 1H01459）。
