Center of Deep-Sea Research & CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, PR China.
Int J Syst Evol Microbiol. 2022 Feb;72(2). doi: 10.1099/ijsem.0.005199.
A moderately halophilic bacterium, designated strain KX20305, was isolated from sediment collected from a cold seep field in the South China Sea. Cells of strain KX20305 were Gram-stain-negative, rod-shaped, non-motile, facultatively anaerobic, oxidase- and catalase-positive, and grew optimally at 25-30 °C, pH 6.0-8.0 and with 3-6 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KX20305 grouped with members of the genus , including D-24 (98.3 % sequence similarity), KMM 3516 (98.1 %) and CC-CZW007 (97.5 %). Genome sequencing of strain KX20305 revealed a genome size of 3.35 Mb and a DNA G+C content of 38.71 mol%. Genomic average nucleotide identity (orthoANI) values of strain KX20305 with D-24, KMM 3516 and JCM 30378 were 83.8, 81.7 and 75.4 %, respectively, while DNA-DNA hybridization (GGDC) values for strain KX20305 with these strains were 27.2, 25.0 and 19.6 %, respectively. The major fatty acids of strain KX20305 were iso-C, iso-C 3-OH and 10-methyl C/iso-C 9. The predominant respiratory quinone was menaquinone-6 (MK-6). The polar lipids mainly comprised phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. Based on comparative analysis of phylogenetic, phylogenomic, phenotypic and chemotaxonomic characteristics, strain KX20305 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KX20305 (=KCTC 82699=MCCC 1K06238=JCM 34635).
从南海冷泉场采集的沉积物中分离到一株中度嗜盐细菌，命名为 KX20305 株。KX20305 菌株的细胞革兰氏阴性，杆状，不运动，兼性厌氧，氧化酶和过氧化氢酶阳性，最适生长温度为 25-30°C，pH 值为 6.0-8.0，盐度为 3-6%（w/v）。基于 16S rRNA 基因序列的系统发育分析表明，KX20305 株与属内的成员聚在一起，包括 D-24（98.3%序列相似性）、KMM 3516（98.1%）和 CC-CZW007（97.5%）。KX20305 株的全基因组测序揭示了一个 3.35Mb 的基因组大小和 38.71mol%的 DNA G+C 含量。KX20305 株与 D-24、KMM 3516 和 JCM 30378 的基因组平均核苷酸同一性（orthoANI）值分别为 83.8%、81.7%和 75.4%，而与这些菌株的 DNA-DNA 杂交（GGDC）值分别为 27.2%、25.0%和 19.6%。KX20305 株的主要脂肪酸为 iso-C、iso-C 3-OH 和 10-甲基 C/iso-C 9。主要的呼吸醌为menaquinone-6（MK-6）。极性脂主要由磷脂酰乙醇胺、两种未鉴定的胺脂和两种未鉴定的脂组成。基于系统发育、系统发育基因组学、表型和化学分类特征的比较分析，KX20305 株代表了属的一个新种，建议将其命名为 sp. nov.。模式菌株为 KX20305（=KCTC 82699=MCCC 1K06238=JCM 34635）。
