Department of Biochemistry, College of Science, King Saud University, P.O.Box 2455, Riyadh, 11451, Saudi Arabia.
Department of Botany, Division of Science and Technology, University of Education, Lahore, Pakistan.
BMC Plant Biol. 2024 Jul 13;24(1):667. doi: 10.1186/s12870-024-05359-z.
Recent studies have exhibited a very promising role of copper nanoparticles (CuNPs) in mitigation of abiotic stresses in plants. Arbuscular mycorrhizae fungi (AMF) assisted plants to trigger their defense mechanism against abiotic stresses. Arsenic (As) is a non-essential and injurious heavy-metal contaminant. Current research work was designed to elucidate role of CuNPs (100, 200 and 300 mM) and a commercial inoculum of Glomus species (Clonex Root Maximizer) either alone or in combination (CuNPs + Clonex) on physiology, growth, and stress alleviation mechanisms of E. sibiricus growing in As spiked soils (0, 50, and 100 mg Kg soil). Arsenic induced oxidative stress, enhanced biosynthesis of hydrogen peroxide, lipid peroxidation and methylglyoxal (MG) in E. sibiricus. Moreover, As-phytotoxicity reduced photosynthetic activities and growth of plants. Results showed that individual and combined treatments, CuNPs (100 mM) as well as soil inoculation of AMF significantly enhanced root growth and shoot growth by declining As content in root tissues and shoot tissues in As polluted soils. E. sibiricus plants treated with CuNPs (100 mM) and/or AMF alleviated As induced phytotoxicity through upregulating the activity of antioxidative enzymes such as catalase (CAT) and superoxide dismutase (SOD) besides the biosynthesis of non-enzymatic antioxidants including phytochelatin (PC) and glutathione (GSH). In brief, supplementation of CuNPs (100 mM) alone or in combination with AMF reduced As uptake and alleviated the As-phytotoxicity in E. sibiricus by inducing stress tolerance mechanism resulting in the improvement of the plant growth parameters.
最近的研究表明，铜纳米粒子（CuNPs）在减轻植物非生物胁迫方面具有非常有前景的作用。丛枝菌根真菌（AMF）可以帮助植物触发其对非生物胁迫的防御机制。砷（As）是一种非必需的、有害的重金属污染物。本研究旨在阐明 CuNPs（100、200 和 300mM）和 Glomus 种的商业接种剂（Clonex Root Maximizer）单独或联合（CuNPs+Clonex）在受 As 污染土壤（0、50 和 100mg Kg 土壤）中生长的西伯利亚赤杨的生理、生长和缓解压力机制中的作用。砷诱导氧化应激，增强过氧化氢、脂质过氧化和甲基乙二醛（MG）的生物合成，从而导致西伯利亚赤杨受到胁迫。此外，As 对植物的毒性降低了光合作用和植物的生长。结果表明，单独和联合处理，CuNPs（100mM）以及 AMF 土壤接种均显著增强了根和茎的生长，降低了受 As 污染土壤中根系和地上部组织中的 As 含量。用 CuNPs（100mM）和/或 AMF 处理的西伯利亚赤杨通过上调抗氧化酶（如过氧化氢酶（CAT）和超氧化物歧化酶（SOD））的活性以及非酶抗氧化剂（如谷胱甘肽（GSH））的生物合成，缓解了 As 诱导的植物毒性。总之，单独或与 AMF 联合补充 CuNPs（100mM）可以减少 As 的吸收，并通过诱导胁迫耐受机制减轻西伯利亚赤杨的 As 毒性，从而改善植物生长参数。
