Miltenburger H G
Dev Biol Stand. 1985;60:147-59.
The evaluation of sublethal cellular damage is shown to be important for the performance of experiments and bioproduction with animal cells. Detailed information on the fate of single cells in colony pedigrees from untreated and irradiated mammalian cells was obtained in vitro with cinematography recordings. It can be demonstrated that in generations two, three, and four the cells are more sensitive because of higher death rates and increased generation times induced by the handling of the cells during passaging or by the damaging agent. It is obvious that chemical agents can act in the same way. Also poor medium quality induces similar effects. Growth curves of single colony cell populations render much better information on the proliferation of a cell population than the determination of cells per ml (= particle counting). Examples are given for such growth curves and for colony size distribution curves based on individual cell countings. The advantages of the use of flow-cytofluorometry for the control of cell cycle phases are shown. As an example for the relevance of qualitative and quantitative data on cell substrate and medium conditions for bioproduction the in vitro mass replication of an insect pathogenic virus (baculovirus) is presented. Baculoviruses are potentially important biological pesticides which can replace chemical insecticides partially.
亚致死性细胞损伤的评估对于动物细胞实验和生物生产的开展具有重要意义。通过电影摄影记录在体外获得了未经处理和经辐射的哺乳动物细胞集落谱系中单个细胞命运的详细信息。可以证明，在传代后的第二代、第三代和第四代中，由于传代过程中细胞处理或损伤剂导致的较高死亡率和延长的代时，细胞更为敏感。显然，化学试剂也能产生同样的作用。同样，培养基质量不佳也会引发类似效应。单细胞群体的生长曲线比每毫升细胞计数（=颗粒计数）能提供关于细胞群体增殖的更好信息。文中给出了此类生长曲线以及基于单个细胞计数的集落大小分布曲线的示例。展示了使用流式细胞荧光术控制细胞周期阶段的优势。作为细胞底物和培养基条件的定性和定量数据对生物生产相关性的一个例子，介绍了一种昆虫致病病毒（杆状病毒）的体外大量复制。杆状病毒是潜在的重要生物杀虫剂，可部分替代化学杀虫剂。
