Himmler G, Palfi G, Rüker F, Katinger H, Scheirer W
Dev Biol Stand. 1985;60:291-6.
Mass culture of immobilized cells in airlift-fermenters usually ends up with the beads accumulating in the foamy layer on the surface of the reactor fluid or, in stirred tankreactors, with partial destruction of the beads. We tried to use an airlift fermenter vessel for growing cells, immobilized in agarose beads. Instead of using the gas for driving, we mounted a slowly turning marine type impeller within the drought tube. Oxygen was supplied on occasional demands by the original sparger. This set up leads to sufficient operational characteristics of the reactor without accumulation of the beads in the foamy layer and without mechanical destruction. Different productivities of either immobilized cells or cells in free suspension culture are reported.
在气升式发酵罐中对固定化细胞进行大规模培养，通常会导致珠子堆积在反应器流体表面的泡沫层中，或者在搅拌罐式反应器中，珠子会部分受损。我们尝试使用气升式发酵罐来培养固定在琼脂糖珠中的细胞。我们没有使用气体驱动，而是在干燥管内安装了一个缓慢旋转的船用型叶轮。通过原来的气体分布器按需供应氧气。这种设置使反应器具有足够的运行特性，珠子不会堆积在泡沫层中，也不会受到机械破坏。报告了固定化细胞或游离悬浮培养细胞的不同生产力。
