[The cultivation of hybridoma cells in agarose granules].
作者信息
Kukhareva L V, D'iakonov I A, Blinova M I, Nikolaenko N S, Pinaev G P
出版信息
Tsitologiia. 1990;32(4):364-70.
PMID:2238110
Abstract
The results of cultivation of agarose gel immobilized hybridoma cells producing monoclonal antibodies to human alpha 2-interferon are presented. The immobilized cultured cells retained the higher viability for 10-28 days without changing the growth medium, in comparison with cultured cells in control suspension. The cells immobilized in agarose gel demonstrated a tendency to polyploidization as was revealed by the technique of DNA cytofluorimetry with Hoechst 33258.
摘要
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[The cultivation of hybridoma cells in agarose granules].
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