Velan B, Cohen S, Grosfeld H, Shalita Z, Shafferman A
Dev Biol Stand. 1985;60:355-69.
Bovine peripheral leukocytes were virally induced for interferon production, and an acid stable, SDS stable, antiviral activity was detected in the preparation. This bovine interferon (BoIFN) was tested for its ability to induce an antiviral state in various mammalian cells and was found to be specific to cells from bovine origin. The BoIFN cross reacts with antibodies against human IFN-alpha but these antibodies do not neutralize the bovine IFN activity. Leukocyte BoIFN exhibits polymorphism upon Affi-Gel Blue chromatography and SDS-PAGE (16k and 24K). The virally induced leukocytes produce a 13S mRNA which upon translation in oocytes yields an active IFN molecule. Bovine genomic library was constructed and screened for BoIFN-alpha sequences, using human IFN-alpha probes. From the clones isolated, five were found to represent distinct genes. Sequence analysis indicate that these genes are closely related (94% homology). One of these genes was expressed in E. coli under the control of trp promoter operator. The physicochemical and biological properties of the bacterial BoIFN-alpha product resemble those of a subpopulation of natural BoIFN.
牛外周血白细胞经病毒诱导产生干扰素，在制备物中检测到一种耐酸、耐十二烷基硫酸钠的抗病毒活性。对这种牛干扰素（BoIFN）诱导各种哺乳动物细胞产生抗病毒状态的能力进行了测试，发现它对牛源细胞具有特异性。BoIFN与抗人IFN-α抗体发生交叉反应，但这些抗体不能中和牛干扰素的活性。白细胞BoIFN在Affi-Gel Blue柱层析和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）（16k和24K）上呈现多态性。经病毒诱导的白细胞产生一种13S信使核糖核酸（mRNA），在卵母细胞中翻译后产生一种活性干扰素分子。构建了牛基因组文库，并用人类IFN-α探针筛选BoIFN-α序列。从分离出的克隆中，发现有五个代表不同的基因。序列分析表明这些基因密切相关（同源性为94%）。其中一个基因在色氨酸启动子操纵子的控制下在大肠杆菌中表达。细菌产生的BoIFN-α产物的物理化学和生物学特性与天然BoIFN的一个亚群相似。
