Linnane A W, Beilharz M W, McMullen G L, Macreadie I G, Murphy M, Nisbet I T, Novitski C E, Woodrow G C
Biochem Int. 1984 May;8(5):725-32.
The complete nucleotide sequence of a human interferon-alpha gene is reported. The gene, designated IFN-alpha M1, was isolated from a human genomic library in phage lambda Charon 4A using synthetic oligonucleotides as hybridization probes. Based on a comparison of nucleotide sequence data obtained from this recombinant phage with published interferon-alpha gene sequences, a region of DNA capable of coding for a pre-interferon of 189 amino acids was identified. An AluI fragment containing the coding region for the mature interferon was inserted into the HincII site of the phage M13mp11, resulting in a fusion of portions of the IFN-alpha M1 and the beta-galactosidase genes. Antiviral activity was detected in extracts from E. coli infected with the recombinant M13 phage carrying the fused gene. The antiviral activity was completely neutralized by antibodies to human interferon-alpha.
报道了一个人α-干扰素基因的完整核苷酸序列。该基因命名为IFN-αM1，是用合成寡核苷酸作为杂交探针从噬菌体λCharon 4A的人基因组文库中分离出来的。根据从这个重组噬菌体获得的核苷酸序列数据与已发表的α-干扰素基因序列进行比较，确定了一个能够编码189个氨基酸的前干扰素的DNA区域。将含有成熟干扰素编码区的AluI片段插入噬菌体M13mp11的HincII位点，导致IFN-αM1和β-半乳糖苷酶基因部分融合。在用携带融合基因的重组M13噬菌体感染的大肠杆菌提取物中检测到抗病毒活性。该抗病毒活性被抗人α-干扰素抗体完全中和。
