Weismann C, Nagata S, Boll S, Fountoulakis M, Fujisawa A, Fujisawa J, Haynes J, Henco K, Mantei N, Ragg H
Princess Takamatsu Symp. 1982;12:1-22.
cDNA was prepared from induced leukocyte poly(A) RNA and cloned in Escherichia coli. Interferon (IFN)-alpha cDNA clones were isolated by subculture cloning using a translation hybridization assay. Definitive identification of the clones was based on the production of an interferon-like protein by the transformed bacteria. Different IFN-alpha cDNAs, with characteristic target cell specificities, were identified. The cloned cDNAs typically encode a mature polypeptide of 166 (or, in the case of IFN-alpha 2, 165) amino acids and a signal sequence of 23 amino acids.
从诱导的白细胞多聚腺苷酸RNA制备cDNA，并克隆到大肠杆菌中。使用翻译杂交试验通过亚克隆分离干扰素（IFN）-α cDNA克隆。克隆的最终鉴定基于转化细菌产生的干扰素样蛋白。鉴定出具有特征性靶细胞特异性的不同IFN-α cDNA。克隆的cDNA通常编码一个由166个（对于IFN-α 2则为165个）氨基酸组成的成熟多肽和一个由23个氨基酸组成的信号序列。
