Yang Jie, Bao Zhiyuan, Li Jiali, Lu Tingting, Cai Jiawei, Sun Shaoning, Shen Ning, Chen Yang, Zhao Bohao, Wu Xinsheng
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China.
Animals (Basel). 2024 Jun 29;14(13):1930. doi: 10.3390/ani14131930.
Litter size is a significant economic trait during animal reproduction. This current study attempted to decipher whether promotes the apoptosis of granulosa cells (GCs) and inhibits their proliferation by investigating the effects of the gene using flow cytometry and a Cell Counting Kit-8 (CCK-8) assay. is linked with ovarian follicle development in the reproductive performance of 104 female New Zealand rabbits. We observed that could regulate the mRNA of follicular development-related genes (, , , , , and ) with a qRT-PCR, and we observed the protein expression of CITED1 and GHR using a western blot (WB) analysis. The dual luciferase activity assays helped identify the core promoter region of the gene, and the polymorphism of the promoter region was studied using Sanger sequencing. The results indicated four single nucleotide polymorphisms (SNPs) within the core promoter region, among which the g.-680C>A locus was significantly associated with both the total and alive litter sizes. Additionally, the CC genotype was associated with the largest total and alive litter sizes, compared to the CA and AA genotypes ( < 0.05). In conclusion, this study investigated the effects of on ovarian granulosa cells and its association with selected reproductive parameters in rabbits. The results provide a theoretical foundation for the use of as a molecular marker in rabbits.
产仔数是动物繁殖过程中的一个重要经济性状。本研究试图通过流式细胞术和细胞计数试剂盒-8（CCK-8）检测法研究该基因的作用，以破译其是否促进颗粒细胞（GCs）凋亡并抑制其增殖。该基因与104只雌性新西兰兔繁殖性能中的卵泡发育有关。我们通过qRT-PCR观察到该基因可调节卵泡发育相关基因（、、、、和）的mRNA，并通过蛋白质免疫印迹（WB）分析观察到CITED1和GHR的蛋白表达。双荧光素酶活性测定有助于鉴定该基因的核心启动子区域，并使用桑格测序法研究该基因启动子区域的多态性。结果表明，核心启动子区域内存在四个单核苷酸多态性（SNPs），其中g.-680C>A位点与总产仔数和存活产仔数均显著相关。此外，与CA和AA基因型相比，CC基因型与最大的总产仔数和存活产仔数相关（<0.05）。总之，本研究调查了该基因对兔卵巢颗粒细胞的影响及其与所选繁殖参数的关联。研究结果为将该基因用作兔的分子标记提供了理论基础。
