Kovács Dóra, Palkovicsné Pézsa Nikolett, Móritz Alma Virág, Jerzsele Ákos, Farkas Orsolya
Department of Pharmacology and Toxicology, University of Veterinary Medicine, 1078 Budapest, Hungary.
National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, 1078 Budapest, Hungary.
Animals (Basel). 2024 Jul 2;14(13):1952. doi: 10.3390/ani14131952.
Intestinal infections caused by and pose a huge economic burden on the swine industry that is exacerbated by the development of antimicrobial resistance in these pathogens, thus raising the need for alternative prevention and treatment methods. Our aim was to test the beneficial effects of the flavonoid luteolin in an in vitro model of porcine intestinal infections. We infected the porcine intestinal epithelial cell line IPEC-J2 with and . subsp. serovar Typhimurium (10 CFU/mL) with or without previous, concurrent, or subsequent treatment with luteolin (25 or 50 µg/mL), and measured the changes in the reactive oxygen species and interleukin-6 and -8 levels of cells. We also tested the ability of luteolin to inhibit the adhesion of bacteria to the cell layer, and to counteract the barrier integrity damage caused by the pathogens. Luteolin was able to alleviate oxidative stress, inflammation, and barrier integrity damage, but it could not inhibit the adhesion of bacteria to IPEC-J2 cells. Luteolin is a promising candidate to be used in intestinal infections of pigs, however, further studies are needed to confirm its efficacy. The use of luteolin in the future could ultimately lead to a reduced need for antibiotics in pig production.
由[具体病原体名称1]和[具体病原体名称2]引起的肠道感染给养猪业带来了巨大的经济负担，而这些病原体中抗菌药物耐药性的发展使这种负担进一步加剧，因此需要替代性的预防和治疗方法。我们的目的是在猪肠道感染的体外模型中测试类黄酮木犀草素的有益作用。我们用[具体病原体名称1]和[具体病原体名称2]感染猪肠道上皮细胞系IPEC-J2。用鼠伤寒沙门氏菌亚种鼠伤寒血清型（10 CFU/mL）感染细胞，在感染前、感染期间或感染后用木犀草素（25或50 µg/mL）进行处理，然后测量细胞中活性氧、白细胞介素-6和-8水平的变化。我们还测试了木犀草素抑制细菌黏附到细胞层以及对抗病原体对屏障完整性造成损害的能力。木犀草素能够减轻氧化应激、炎症反应和屏障完整性损害，但它不能抑制细菌黏附到IPEC-J2细胞上。木犀草素是用于猪肠道感染的一个有前景的候选药物，然而，需要进一步研究来证实其疗效。未来使用木犀草素最终可能会减少养猪生产中对抗生素的需求。
