Palkovicsné Pézsa Nikolett, Kovács Dóra, Rácz Bence, Farkas Orsolya
Department of Pharmacology and Toxicology, University of Veterinary Medicine Budapest, 1078 Budapest, Hungary.
Department of Anatomy and Histology, University of Veterinary Medicine Budapest, 1078 Budapest, Hungary.
Microorganisms. 2022 Apr 29;10(5):936. doi: 10.3390/microorganisms10050936.
The emergence of antimicrobial resistance raises serious concerns worldwide. Probiotics offer a promising alternative to enhance growth promotion in farm animals; however, their mode of action still needs to be elucidated. The IPEC-J2 cell line (porcine intestinal epithelial cells) is an appropriate tool to study the effect of probiotics on intestinal epithelial cells. In our experiments, IPEC-J2 cells were challenged by two gastrointestinal (GI) infection causing agents, () or ser. Typhimurium ( Typhimurium). We focused on determining the effect of pre-, co-, and post-treatment with two probiotic candidates, or , on the barrier function, proinflammatory cytokine (IL-6 and IL-8) response, and intracellular reactive oxygen species (ROS) production of IPEC-J2 cells, in addition to the adhesion inhibition effect. ( and ( proved to be anti-inflammatory and had an antioxidant effect under certain treatment combinations, and further effectively inhibited the adhesion of pathogenic bacteria. Interestingly, they had little effect on paracellular permeability. Based on our results, and are both promising candidates to contribute to the beneficial effects of probiotic multispecies mixtures.
抗菌药物耐药性的出现引起了全球的严重关注。益生菌为促进农场动物生长提供了一种有前景的替代方法；然而，它们的作用方式仍有待阐明。IPEC-J2细胞系（猪肠道上皮细胞）是研究益生菌对肠道上皮细胞作用的合适工具。在我们的实验中，IPEC-J2细胞受到两种胃肠道感染致病因子（）或鼠伤寒沙门氏菌血清型鼠伤寒沙门氏菌（）的攻击。我们重点研究了两种候选益生菌（或）预处理、共处理和后处理对IPEC-J2细胞的屏障功能、促炎细胞因子（IL-6和IL-8）反应、细胞内活性氧（ROS）产生的影响，以及黏附抑制作用。（和（在某些处理组合下被证明具有抗炎和抗氧化作用，并进一步有效抑制病原菌的黏附。有趣的是，它们对细胞旁通透性影响很小。基于我们的结果，和都是有望为益生菌多物种混合物的有益效果做出贡献的候选者。
