Department of Pharmacology and Toxicology, University of Veterinary Medicine Budapest, 1078 Budapest, Hungary.
Department of Anatomy and Histology, University of Veterinary Medicine Budapest, 1078 Budapest, Hungary.
Nutrients. 2022 Apr 2;14(7):1486. doi: 10.3390/nu14071486.
In farm animals, intestinal diseases caused by spp. and may lead to significant economic loss. In the past few decades, the swine industry has largely relied on the prophylactic use of antibiotics to control gastrointestinal diseases. The development of antibiotic resistance has become an important issue both in animal and human health. The use of antibiotics for prophylactic purposes has been banned, moreover the new EU regulations further restrict the application of antibiotics in veterinary use. The swine industry seeks alternatives that are capable of maintaining the health of the gastrointestinal tract. Probiotics offer a promising alternative; however, their mode of action is not fully understood. In our experiments, porcine intestinal epithelial cells (IPEC-J2 cells) were challenged by Typhimurium or and we aimed at determining the effect of pre-, co-, and post-treatment with NCIMB 10415 on the internal redox state, paracellular permeability, IL-6 and IL-8 secretion of IPEC-J2 cells. Moreover, the adhesion inhibition effect was also investigated. was able to reduce oxidative stress and paracellular permeability of IPEC-J2 cells and could inhibit the adhesion of and . Based on our results, is a promising candidate to maintain the health of the gastrointestinal tract.
在农场动物中，由 spp. 和 引起的肠道疾病可能导致重大的经济损失。在过去几十年中，养猪业在很大程度上依赖于预防性使用抗生素来控制胃肠道疾病。抗生素耐药性的发展已成为动物和人类健康的一个重要问题。出于预防目的使用抗生素已被禁止，此外，欧盟的新法规进一步限制了抗生素在兽医中的应用。养猪业正在寻找能够维持胃肠道健康的替代方法。益生菌提供了一种有前途的替代方法；然而，其作用机制尚不完全清楚。在我们的实验中，猪肠上皮细胞（IPEC-J2 细胞）受到肠炎沙门氏菌或大肠杆菌的挑战，我们旨在确定用 NCIMB 10415 进行预处理、共处理和后处理对 IPEC-J2 细胞内部氧化还原状态、细胞旁通透性、IL-6 和 IL-8 分泌的影响。此外，还研究了其抑制黏附的效果。结果表明， 能够降低 IPEC-J2 细胞的氧化应激和细胞旁通透性，并能抑制 和 的黏附。基于我们的结果， 是维持胃肠道健康的有前途的候选物。
