Al-Sudani Waleed Khaled Kaddem, Al-Shammari Rawaa Shakir Shnain, Abed Mohammed Saheb, Al-Saedi Jasim Hafedh, Mernea Maria, Lungu Iulia Ioana, Dumitrache Florian, Mihailescu Dan Florin
Interdisciplinary School of Doctoral Studies, University of Bucharest, 36-46 Mihail Kogălniceanu Bd, 050107 Bucharest, Romania.
Ministry of Trade in Iraq, The General Company for Foodstuff Trade, Al Mansour, Baghdad 10013, Iraq.
Plants (Basel). 2024 Jun 21;13(13):1724. doi: 10.3390/plants13131724.
The enhancement of seed germination by using nanoparticles (NPs) holds the potential to elicit the synthesis of more desired compounds with important biomedical applications, such as preventing protein glycation, which occurs in diabetes. Here, we used 7 nm and 100 nm ZnO and 4.5 nm and 16.7 nm FeO NPs to treat sunflower seeds. We evaluated the effects on germination, total phenolic content, and the anti-glycation potential of extracted polyphenols. Sunflower seeds were allowed to germinate in vitro after soaking in NP solutions of different concentrations. Polyphenols were extracted, dosed, and used in serum albumin glycation experiments. The germination speed of seeds was significantly increased by the 100 nm ZnO NPs and significantly decreased by the 4.5 nm FeO NPs. The total phenolic content (TPC) of seeds was influenced by the type of NP, as ZnO NPs enhanced TPC, and the size of the NPs, as smaller NPs led to improved parameters. The polyphenols extracted from seeds inhibited protein glycation, especially those extracted from seeds treated with 7 nm ZnO. The usage of NPs impacted the germination speed and total polyphenol content of sunflower seeds, highlighting the importance of NP type and size in the germination process.
使用纳米颗粒（NPs）促进种子萌发具有引发合成更多具有重要生物医学应用的所需化合物的潜力，例如预防糖尿病中发生的蛋白质糖基化。在此，我们使用了7纳米和100纳米的氧化锌纳米颗粒以及4.5纳米和16.7纳米的氧化亚铁纳米颗粒来处理向日葵种子。我们评估了其对萌发、总酚含量以及提取的多酚的抗糖基化潜力的影响。将向日葵种子浸泡在不同浓度的纳米颗粒溶液中后进行体外萌发。提取多酚，进行定量，并用于血清白蛋白糖基化实验。100纳米的氧化锌纳米颗粒显著提高了种子的萌发速度，而4.5纳米的氧化亚铁纳米颗粒则显著降低了种子的萌发速度。种子的总酚含量（TPC）受纳米颗粒类型的影响，氧化锌纳米颗粒提高了TPC，同时也受纳米颗粒尺寸的影响，较小的纳米颗粒使各项参数得到改善。从种子中提取的多酚抑制了蛋白质糖基化，尤其是从用7纳米氧化锌处理的种子中提取的多酚。纳米颗粒的使用影响了向日葵种子的萌发速度和总多酚含量，突出了纳米颗粒类型和尺寸在萌发过程中的重要性。
