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生物成因纳米粒子对罗勒(Ocimum basilicum L.)种子萌发的刺激作用。

Stimulating effect of biogenic nanoparticles on the germination of basil (Ocimum basilicum L.) seeds.

机构信息

Department of Chemical Engineering, Suleyman Demirel University, 32260, Isparta, Turkey.

Department of Biology, Suleyman Demirel University, 32260, Isparta, Turkey.

出版信息

Sci Rep. 2024 Jan 19;14(1):1715. doi: 10.1038/s41598-023-50654-8.

DOI:10.1038/s41598-023-50654-8
PMID:38242902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10798979/
Abstract

Metal nanoparticles synthesized using various biosources are the subject of focus in many research areas thanks to their improved biological effects and increased bioavailability. Silver (Ag), zinc oxide (ZnO) and magnetite (FeO) nanoparticles (NPs) were obtained by using low-cost, low-energy, environmentally friendly, non-toxic chemicals and easily accessible thyme leaves and lavender flowers. The effects of various concentrations of biosynthesized NPs on the germination and germination index of basil seeds were defined comparatively. Phytochemicals in lavender flower extract acted as reducing and capping agents in the biosynthesis of Ag-NPs, and phytochemicals in thyme leaves extract acted for the biosynthesis of ZnO-NPs ve FeO-NPs. Relative root length was detected at 25 mg/L ZnO-NP, stem length at 50 mg/L ZnO-NP, and relative seed germination 100 mg/L FeO-NP with the maximum value. However, germination percentage, germination index, germination vigor index and root length were found to be maximum compared to other NP applications at Ag-NPs at 200 mg/L. This research showed that the germination promoting effects of NPs, which may be essential microelements, are related to their size, surface area, morphology and concentration. Thus, it promoted early and rapid germination by breaking the NP's seed dormancy.

摘要

使用各种生物源合成的金属纳米粒子因其改善的生物效应和提高的生物利用度而成为许多研究领域的关注焦点。银(Ag)、氧化锌(ZnO)和磁铁矿(FeO)纳米粒子(NPs)是通过使用低成本、低能量、环保、无毒的化学品和易于获得的百里香叶和薰衣草花合成的。比较了不同浓度的生物合成 NPs 对罗勒种子发芽和发芽指数的影响。薰衣草花提取物中的植物化学物质在 Ag-NPs 的生物合成中充当还原剂和封端剂,而百里香叶提取物中的植物化学物质则用于 ZnO-NPs 和 FeO-NPs 的生物合成。在 25mg/L ZnO-NP 下检测到相对根长,在 50mg/L ZnO-NP 下检测到茎长,在 100mg/L FeO-NP 下检测到相对种子发芽率,达到最大值。然而,与其他 NP 应用相比,Ag-NPs 在 200mg/L 时的发芽率、发芽指数、发芽活力指数和根长达到最大值。这项研究表明,纳米粒子的促进发芽作用可能与其大小、表面积、形态和浓度有关,是必需的微量元素。因此,它通过打破 NP 的种子休眠来促进早期和快速发芽。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd88/10798979/086462c5626b/41598_2023_50654_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd88/10798979/4ebea21fb87b/41598_2023_50654_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd88/10798979/5bf5e23305b4/41598_2023_50654_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd88/10798979/84a074937f2b/41598_2023_50654_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd88/10798979/086462c5626b/41598_2023_50654_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd88/10798979/4ebea21fb87b/41598_2023_50654_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd88/10798979/5bf5e23305b4/41598_2023_50654_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd88/10798979/84a074937f2b/41598_2023_50654_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd88/10798979/086462c5626b/41598_2023_50654_Fig4_HTML.jpg

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