Lu Hejun, Xiao Yuyang, Liu Yuxin, Zhang Jiachen, Zhao Yanyan
Institute of Crop Science, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
Xianghu Laboratory, Hangzhou 311231, China.
Plants (Basel). 2024 Jun 28;13(13):1789. doi: 10.3390/plants13131789.
Leaf color mutants serve as ideal materials for studying photosynthesis, chlorophyll metabolism, and other physiological processes. Here, we identified a spontaneous yellow-leaf mutant () with chlorophyll-reduced leaves from L. cv ZM24. Compare to wild type ZM24 with green leaves, exhibited patchy yellow leaves and reduced chlorophyll content. To further explore the mechanisms of the patchy yellow phenotype of the mutant plant, the transcriptomics and proteomics profiles were conducted for the mutant and wild types. A total of 9247 differentially expressed genes (DEGs) and 1368 differentially accumulated proteins (DAPs) were identified. Following gene ontology (GO) annotation and KEGG enrichment, the DEGs/DAPs were found to be significantly involved in multiple important pathways, including the obsolete oxidation-reduction process, photosynthesis, light-harvesting, the microtubule-based process, cell redox homeostasis, and the carbohydrate metabolic process. In photosynthesis and the light-harvesting pathway, a total of 39 DAPs/DEGs were identified, including 9 genes in the PSI, 7 genes in the PS II, 9 genes in the light-harvesting chlorophyll protein complex (LHC), 10 genes in the PsbP family, and 4 genes in the cytochrome b6/f complex. To validate the reliability of the omics data, GhPPD1, a DAPs in the PsbP family, was knocked down in cotton using the TRV-based VIGS system, and it was observed that the -silenced plants exhibited patchy yellow color, accompanied by a significant decrease in chlorophyll content. In conclusion, this study integrated transcriptomic and proteomic approaches to gain a deeper understanding of the mechanisms underlying the chlorophyll-reduced leaf phenotype.
叶色突变体是研究光合作用、叶绿素代谢及其他生理过程的理想材料。在此，我们从陆地棉品种ZM24中鉴定出一个自发黄叶突变体（），其叶片叶绿素含量降低。与绿叶野生型ZM24相比，表现出斑驳黄叶且叶绿素含量降低。为进一步探究突变体植株斑驳黄化表型的机制，对突变体和野生型进行了转录组学和蛋白质组学分析。共鉴定出9247个差异表达基因（DEGs）和1368个差异积累蛋白（DAPs）。经过基因本体（GO）注释和KEGG富集分析，发现这些DEGs/DAPs显著参与多个重要途径，包括过时的氧化还原过程、光合作用、光捕获、基于微管的过程、细胞氧化还原稳态和碳水化合物代谢过程。在光合作用和光捕获途径中，共鉴定出39个DAPs/DEGs，包括光合系统I中的9个基因、光合系统II中的7个基因、光捕获叶绿素蛋白复合体（LHC）中的9个基因、PsbP家族中的10个基因以及细胞色素b6/f复合体中的4个基因。为验证组学数据的可靠性，利用基于TRV的VIGS系统在棉花中敲低PsbP家族中的一个DAPs——GhPPD1，观察到沉默的植株呈现斑驳黄色，同时叶绿素含量显著降低。总之，本研究整合转录组学和蛋白质组学方法，以更深入了解叶绿素含量降低叶片表型的潜在机制。
