Yu Youfeng, Zhu Rui, Xu Hao, Enugutti Balaji, Schneitz Kay, Wang Xuanpeng, Li Jing
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Sanya Institute of Breeding and Multiplication, Hainan University, Sanya 572025, China.
Plants (Basel). 2024 Jul 3;13(13):1824. doi: 10.3390/plants13131824.
Regulation of microtubule dynamics is crucial during key developmental transitions such as gametogenesis, fertilization, embryogenesis, and seed formation, where cells undergo rapid changes in shape and function. In plants, katanin plays an essential role in microtubule dynamics. This study investigates two seed developmental mutants in , named (, ) and (), which are characterized by round seeds, dwarfism, and fertility defects. Notably, exhibits a dominant inheritance pattern, whereas is recessive. Through positional cloning, we identified both mutants as new alleles of the () gene, which encodes a microtubule-severing enzyme critical for cell division and morphology. Mutations in disrupt embryo cell division and lead to the emergence of a twin embryo phenotype. Our findings underscore the essential role of KTN1 in fertility and early embryonic development, potentially influencing the fate of reproductive cells.
微管动力学的调控在关键发育转变过程中至关重要，如配子发生、受精、胚胎发生和种子形成，在此过程中细胞的形状和功能会发生快速变化。在植物中，katanin在微管动力学中发挥着重要作用。本研究调查了两种种子发育突变体，命名为（，）和（），其特征为圆形种子、矮化和育性缺陷。值得注意的是，表现出显性遗传模式，而则是隐性的。通过定位克隆，我们将这两种突变体鉴定为（）基因的新等位基因，该基因编码一种对细胞分裂和形态至关重要的微管切断酶。中的突变会破坏胚胎细胞分裂并导致双胚表型的出现。我们的研究结果强调了KTN1在育性和早期胚胎发育中的重要作用，可能影响生殖细胞的命运。
