Luptovčiak Ivan, Samakovli Despina, Komis George, Šamaj Jozef
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University OlomoucOlomouc, Czechia.
Front Plant Sci. 2017 May 5;8:728. doi: 10.3389/fpls.2017.00728. eCollection 2017.
Cytoskeletal remodeling has a fundamental role, especially during transitional developmental stages when cells rapidly adopt new forms and roles, like gametogenesis, fertilization and concomitant embryogenesis and seed formation. KATANIN 1, a microtubule severing protein, fulfills a major regulatory mechanism of dynamic microtubule turnover in eukaryotes. Herein, we show that three well-established mutants, and collectively display lower fertility and seed set in Arabidopsis. These lower fertility and seed set rates of and mutants were correlated to abnormalities in the development of embryo proper and seed. Such phenotypes were rescued by transformation of mutants with functional construct. This study significantly expands the already broad functional repertoire of KATANIN 1 and unravels its new role in embryo and seed development. Thus, KATANIN 1 significantly contributes to the fertility and proper embryo and seed formation in Arabidopsis.
细胞骨架重塑具有重要作用，尤其是在过渡发育阶段，此时细胞迅速采用新的形式和功能，如配子发生、受精以及随之而来的胚胎发生和种子形成过程。katanin 1是一种微管切断蛋白，它在真核生物中实现了动态微管周转的主要调节机制。在此，我们表明，三个已被充分证实的突变体，共同表现出较低的育性和种子结实率。这些突变体较低的育性和种子结实率与胚和种子发育异常相关。通过用功能性构建体转化突变体，这些表型得到了挽救。本研究显著扩展了katanin 1已经广泛的功能范围，并揭示了其在胚胎和种子发育中的新作用。因此，katanin 1对拟南芥的育性以及正常的胚胎和种子形成有显著贡献。
