Ma Yongzhen, Li Mengtao, Wang Shujuan, Deng Ke, Zhao Long, Luo Jia, Wang Wenquan, Wang Fang, Wang Jian
Qinghai University, Xining 810016, China.
Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China.
Plants (Basel). 2024 Jul 8;13(13):1879. doi: 10.3390/plants13131879.
The timing of potato tuberization is affected by potato ripeness, environmental factors, and polygene regulation. The accurate control of the transition to tuberization has both scientific and practical production value, but the key factors regulating this transition remain unclear. This study grafted an early-maturing potato variety (Favorita) scion to the late-maturing Qingshu 9 variety and demonstrated that a heterologous early-maturing scion can induce early potato formation on a late-maturing rootstock. The transcriptome of functional leaves and stolons of grafted plants was comprehensively analyzed and 593 differentially expressed genes (DEGs) were identified, including 38 transcription factors. Based on gene molecular function analysis and previous reports, we propose that , , , , , and are the key DEGs that induce tuber formation in early- and late-maturing potatoes. The gene was subjected to functional verification. The leaf area of -overexpressing plants was smaller than the wild type and no potato tubercles were formed, while an RNA interference plant line showed no change in leaf area and formed tubers, indicating that has a role in leaf size regulation and tuber formation.
马铃薯块茎形成的时间受马铃薯成熟度、环境因素和多基因调控的影响。精确控制向块茎形成的转变具有科学和实际生产价值，但调节这一转变的关键因素仍不清楚。本研究将早熟马铃薯品种（费乌瑞它）的接穗嫁接到晚熟品种青薯9号上，证明异源早熟接穗可诱导晚熟砧木上提早形成马铃薯。对嫁接植株功能叶和匍匐茎的转录组进行了全面分析，鉴定出593个差异表达基因（DEGs），其中包括38个转录因子。基于基因分子功能分析和先前的报道，我们提出， 、 、 、 、 和 是诱导早熟和晚熟马铃薯块茎形成的关键DEGs。对 基因进行了功能验证。过表达 的植株叶面积小于野生型，未形成马铃薯块茎，而RNA干扰株系叶面积无变化并形成了块茎，表明 在叶片大小调控和块茎形成中起作用。
