Neuroscience Research Center, Department of Medical and Surgical Sciences, University "Magna Graecia", 88100 Catanzaro, Italy.
Institute of Molecular Biology, Department of Medical and Surgical Sciences, University "Magna Graecia", 88100 Catanzaro, Italy.
Int J Mol Sci. 2024 Jun 23;25(13):6882. doi: 10.3390/ijms25136882.
Clinical differentiation of progressive supranuclear palsy (PSP) from Parkinson's disease (PD) is challenging due to overlapping phenotypes and the late onset of specific atypical signs. Therefore, easily assessable diagnostic biomarkers are highly needed. Since PD is a synucleopathy while PSP is a tauopathy, here, we investigated the clinical usefulness of serum oligomeric-α-synuclein (o-α-synuclein) and 181Thr-phosphorylated tau (p-tau181), which are considered as the most important pathological protein forms in distinguishing between these two parkinsonisms. We assessed serum o-α-synuclein and p-tau181 by ELISA and SIMOA, respectively, in 27 PSP patients, 43 PD patients, and 39 healthy controls (HC). Moreover, we evaluated the correlation between serum biomarkers and biological and clinical features of these subjects. We did not find any difference in serum concentrations of p-tau181 and o-α-synuclein nor in the o-α-synuclein/p-tau181 ratio between groups. However, we observed that serum p-tau181 positively correlated with age in HC and PD, while serum o-α-synuclein correlated positively with disease severity in PD and negatively with age in PSP. Finally, the o-α-synuclein/p-tau181 ratio showed a negative correlation with age in PD.
由于重叠的表型和特定非典型体征的出现较晚，所以将进行性核上性麻痹 (PSP) 与帕金森病 (PD) 进行临床区分具有挑战性。因此，非常需要易于评估的诊断生物标志物。由于 PD 是一种突触核蛋白病，而 PSP 是一种 tau 病，因此，我们在这里研究了血清寡聚-α-突触核蛋白 (o-α-synuclein) 和 181Thr 磷酸化 tau (p-tau181) 的临床应用价值，这两种蛋白被认为是区分这两种帕金森病的最重要的病理蛋白形式。我们通过 ELISA 和 SIMOA 分别评估了 27 例 PSP 患者、43 例 PD 患者和 39 名健康对照者 (HC) 的血清 o-α-synuclein 和 p-tau181。此外，我们评估了这些研究对象的血清生物标志物与生物学和临床特征之间的相关性。我们没有发现各组之间 p-tau181 和 o-α-synuclein 的血清浓度以及 o-α-synuclein/p-tau181 比值有任何差异。然而，我们观察到 HC 和 PD 中的血清 p-tau181 与年龄呈正相关，而 PD 中的血清 o-α-synuclein 与疾病严重程度呈正相关，与 PSP 中的年龄呈负相关。最后，PD 中的 o-α-synuclein/p-tau181 比值与年龄呈负相关。
