Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.
Department of Medical, Surgical and Experimental Sciences, University of Sassari, Viale San Pietro 8, 07100 Sassari, Italy.
Int J Mol Sci. 2024 Jun 27;25(13):7026. doi: 10.3390/ijms25137026.
Stem cells possess the ability to differentiate into different lineages and the ability to self-renew, thus representing an excellent tool for regenerative medicine. They can be isolated from different tissues, including the adipose tissue. Adipose tissue and human adipose-derived stem cells (hADSCs) are privileged candidates for regenerative medicine procedures or other plastic reconstructive surgeries. The cellular environment is able to influence the fate of stem cells residing in the tissue. In a previous study, we exposed hADSCs to an exhausted medium of a breast cancer cell line (MCF-7) recovered at different days (4, 7, and 10 days). In the same paper, we inferred that the medium was able to influence the behaviour of stem cells. Considering these results, in the present study, we evaluated the expression of the major genes related to adipogenic and osteogenic differentiation. To confirm the gene expression data, oil red and alizarin red colorimetric assays were performed. Lastly, we evaluated the expression of miRNAs influencing the differentiation process and the proliferation rate, maintaining a proliferative state. The data obtained confirmed that cells exposed to the medium maintained a stem and proliferative state that could lead to a risky proliferative phenotype.
干细胞具有分化为不同谱系和自我更新的能力，因此是再生医学的优秀工具。它们可以从不同的组织中分离出来，包括脂肪组织。脂肪组织和人脂肪来源的干细胞（hADSCs）是再生医学程序或其他整形重建手术的首选候选者。细胞环境能够影响组织中干细胞的命运。在之前的一项研究中，我们将 hADSCs 暴露于从不同天数（4、7 和 10 天）恢复的乳腺癌细胞系（MCF-7）的耗尽培养基中。在同一份论文中，我们推断培养基能够影响干细胞的行为。考虑到这些结果，在本研究中，我们评估了与脂肪生成和成骨分化相关的主要基因的表达。为了证实基因表达数据，进行了油红和茜素红比色测定。最后，我们评估了影响分化过程和增殖率的 miRNA 的表达，维持增殖状态。获得的数据证实，暴露于培养基的细胞保持了一种干细胞和增殖状态，这可能导致危险的增殖表型。
