人脂肪来源干细胞成骨分化过程中差异环状 RNA 表达谱分析。
Differential circular RNA expression profiling during osteogenic differentiation in human adipose-derived stem cells.
机构信息
Department of Plastic Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, PR China.
Department of Stem Cells & Regenerative Medicine, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang, Liaoning 110013, PR China.
出版信息
Epigenomics. 2020 Feb;12(4):289-302. doi: 10.2217/epi-2019-0218. Epub 2020 Feb 13.
Circular RNAs (circRNAs) are essential for stem cell differentiation. This study aimed to investigate their exact mechanism of action in human adipose-derived stem cell (hADSC) osteogenesis. Isolated hADSCs were cultured in growth medium or osteogenic medium, then total RNA was extracted for circRNA microarray, hierarchical cluster, gene ontology, regulating pathway and circRNA-miRNA-mRNA network analyses. A total of 171 circRNAs were upregulated and 119 were downregulated in induced groups compared with those in noninduced groups. Eight circRNAs, 40 miRNAs and 342 mRNAs were selected to construct a competing circRNA-miRNA-mRNA network. These findings may provide novel insight into altered and specific circRNAs that might function as competing endogenous RNAs in hADSCs during osteogenic differentiation.
环状 RNA（circRNAs）在干细胞分化中起着至关重要的作用。本研究旨在探讨其在人脂肪来源干细胞（hADSC）成骨分化中的具体作用机制。分离的 hADSCs 在生长培养基或成骨培养基中培养，然后提取总 RNA 进行 circRNA 微阵列、层次聚类、基因本体论、调控途径和 circRNA-miRNA-mRNA 网络分析。与未诱导组相比，诱导组中有 171 个 circRNAs 上调，119 个 circRNAs 下调。选择了 8 个 circRNAs、40 个 miRNAs 和 342 个 mRNAs 来构建竞争 circRNA-miRNA-mRNA 网络。这些发现可能为 hADSCs 成骨分化过程中作为竞争内源性 RNA 的改变和特定 circRNAs 提供新的见解。
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