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诱导弯曲松茎早期表达的新型 PrMADS11 转录因子的特征。

The Characterization of a Novel PrMADS11 Transcription Factor from Induced Early in Bent Pine Stem.

机构信息

Instituto de Ciencias Biológicas, Universidad de Talca, Av. Lircay s/n, Talca 3465548, Chile.

Facultad de Ciencias Agrarias y Forestales, Universidad Técnica Estatal de Quevedo, Quevedo 120313, Ecuador.

出版信息

Int J Mol Sci. 2024 Jun 30;25(13):7245. doi: 10.3390/ijms25137245.

DOI:10.3390/ijms25137245
PMID:39000352
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11241540/
Abstract

A novel MADS-box transcription factor from D. Don was characterized. encodes a protein of 165 amino acids for a MADS-box transcription factor belonging to group II, related to the MIKC protein structure. was differentially expressed in the stems of pine trees in response to 45° inclination at early times (1 h) was stably transformed with a construct in an effort to identify the putative targets of . A massive transcriptome analysis revealed 947 differentially expressed genes: 498 genes were up-regulated, and 449 genes were down-regulated due to the over-expression of . The gene ontology analysis highlighted a cell wall remodeling function among the differentially expressed genes, suggesting the active participation of cell wall modification required during the response to vertical stem loss. In addition, the phenylpropanoid pathway was also indicated as a target, displaying a marked increment in the expression of the genes driven to the biosynthesis of monolignols. The EMSA assays confirmed that PrMADS11 interacts with CArG-box sequences. This TF modulates the gene expression of several molecular pathways, including other TFs, as well as the genes involved in cell wall remodeling. The increment in the lignin content and the genes involved in cell wall dynamics could be an indication of the key role of PrMADS11 in the response to trunk inclination.

摘要

从 D. Don 中分离出一种新的 MADS-box 转录因子。 编码一个 165 个氨基酸的蛋白质，属于 II 组 MADS-box 转录因子，与 MIKC 蛋白结构相关。 在早期（1 小时）松树茎对 45°倾斜的响应中， 表现出差异表达，为了鉴定 的假定靶标，用 构建体稳定转化了 。大规模转录组分析显示，有 947 个差异表达基因：由于 的过表达，有 498 个基因上调，有 449 个基因下调。基因本体分析突出了差异表达基因中的细胞壁重塑功能，表明在应对垂直茎损失时，细胞壁修饰的积极参与是必需的。此外，还表明苯丙烷途径是 的一个靶标，驱动生物合成木质素单体的基因表达明显增加。EMSA 测定证实 PrMADS11 与 CArG 盒序列相互作用。这种 TF 调节包括其他 TF 在内的几个分子途径的基因表达，以及参与细胞壁重塑的基因。木质素含量的增加和参与细胞壁动力学的基因可能表明 PrMADS11 在树干倾斜响应中的关键作用。

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