Tsampazis Nikolaos, Vavoulidis Eleftherios, Margioula-Siarkou Chrysoula, Symeonidou Marianthi, Intzes Stergios, Papanikolaou Alexios, Dinas Konstantinos, Daniilidis Angelos
2nd Department of Obstetrics & Gynecology, Medical Faculty, Aristotle University of Thessaloniki, Hippokration General Hospital, 56429 Thessaloniki, Greece.
1st Department of Obstetrics & Gynecology, Medical Faculty, Aristotle University of Thessaloniki, Papageorgiou General Hospital, 54124 Thessaloniki, Greece.
Diagnostics (Basel). 2024 Jun 28;14(13):1379. doi: 10.3390/diagnostics14131379.
To evaluate the diagnostic accuracy of Electrical Impedance Spectroscopy (EIS)-assisted colposcopy in detecting CIN2+ Greek women towards standalone colposcopy, HPV mRNA testing, and p16/Ki67 immunostaining.
We conducted a cross-sectional observational study at the Cervical Pathology Clinic of the 2nd Obstetrics-Gynecology University Department of Hippokration Hospital Thessaloniki involving 316 patients from January 2022 to August 2023. All participants provided liquid-based cervical samples for cytology, HPV mRNA testing, and p16/Ki67 immunostaining.
Subsequently, participants underwent both standalone colposcopy and EIS/ZedScan-assisted colposcopy, followed by cervical punch biopsies.
The incorporation of EIS significantly enhanced the sensitivity of colposcopy, increasing it from 54.17% to 100%, equivalent to that of HPV mRNA testing and p16/Ki67 immunostaining, while achieving a high specificity (95.45%). The specificities observed with EIS/ZedScan-assisted and standalone colposcopy were notably superior to those of HPV-related biomarkers (HPV mRNA test and p16/Ki67 immunostaining). When compared to standalone colposcopy, HPV mRNA testing, and p16/Ki67 immunostaining, EIS/ZedScan-assisted colposcopy demonstrated the most favorable combination of Positive and Negative Predictive Values, at 90.57% and 100%, respectively. The inclusion of EIS/ZedScan in colposcopy led to the detection of 44 additional cases of true CIN2+ (100% of the total CIN2+ confirmed histologically) that were missed by standalone colposcopy. This discovery suggests a 45.83% increase in the detection of CIN2+ cases.
The integration of EIS with colposcopy has demonstrated effectiveness in detecting cervical lesions, resulting in a significant detection increase of CIN2+ cases while offering optimal levels of sensitivity, specificity, and predictive values for CIN2+ detection.
评估电阻抗光谱（EIS）辅助阴道镜检查在检测希腊CIN2+女性方面相对于单纯阴道镜检查、HPV mRNA检测和p16/Ki67免疫染色的诊断准确性。
2022年1月至2023年8月，我们在塞萨洛尼基希波克拉底医院第二妇产科大学系的宫颈病理诊所进行了一项横断面观察性研究，纳入了316名患者。所有参与者均提供了用于细胞学检查、HPV mRNA检测和p16/Ki67免疫染色的液基宫颈样本。
随后，参与者接受了单纯阴道镜检查和EIS/ZedScan辅助阴道镜检查，然后进行宫颈活检。
EIS的加入显著提高了阴道镜检查的敏感性，从54.17%提高到100%，与HPV mRNA检测和p16/Ki67免疫染色相当，同时具有较高的特异性（95.45%）。EIS/ZedScan辅助阴道镜检查和单纯阴道镜检查的特异性明显优于HPV相关生物标志物（HPV mRNA检测和p16/Ki67免疫染色）。与单纯阴道镜检查、HPV mRNA检测和p16/Ki67免疫染色相比，EIS/ZedScan辅助阴道镜检查的阳性预测值和阴性预测值组合最为理想，分别为90.57%和100%。在阴道镜检查中加入EIS/ZedScan后，发现了44例单纯阴道镜检查遗漏的真正CIN2+病例（占组织学确诊的CIN2+病例总数的100%）。这一发现表明CIN2+病例的检测率提高了45.83%。
EIS与阴道镜检查相结合已证明在检测宫颈病变方面有效，可显著提高CIN2+病例的检测率，同时为CIN2+检测提供最佳水平的敏感性、特异性和预测值。
