Taussig R, Kaldany R R, Rothbard J B, Schoolnik G, Scheller R H
J Comp Neurol. 1985 Aug 1;238(1):53-64. doi: 10.1002/cne.902380105.
Neuron L11 in the abdominal ganglion of Aplysia californica is thought to be both cholinergic and peptidergic. In previous studies, we isolated a cDNA clone encoding the precursor for an L11 secreted protein(s) by differentially screening an abdominal ganglion cDNA library. We now report the isolation of genomic clones encoding the L11 cDNA sequences. Analysis of these clones reveals that the gene is present in a single copy per haploid genome. RNA blotting and cDNA cloning demonstrate that the L11 gene is expressed not only in the abdominal ganglion but in the head ganglia as well. To define the positions of cells expressing this gene and to follow their processes, we raised antibodies to synthetic peptides defined by the cDNA sequence. Histochemistry revealed about 100 neurons containing immunoreactive material. These cells arborize in the neuropil and are distributed throughout the central nervous system, representing about 0.5% of the Aplysia central neurons. In addition, cells in the abdominal ganglion send processes to the mantle floor at the base of the gill via the genital and branchial nerves. Our data suggest that this network of cells expresses the single L11 peptide gene.
加州海兔腹神经节中的神经元L11被认为既是胆碱能的又是肽能的。在先前的研究中，我们通过差异筛选腹神经节cDNA文库，分离出了一个编码L11分泌蛋白前体的cDNA克隆。我们现在报告编码L11 cDNA序列的基因组克隆的分离。对这些克隆的分析表明，该基因在单倍体基因组中以单拷贝形式存在。RNA印迹和cDNA克隆表明，L11基因不仅在腹神经节中表达，在头神经节中也有表达。为了确定表达该基因的细胞位置并追踪其突起，我们制备了针对由cDNA序列定义的合成肽的抗体。组织化学显示约有100个含有免疫反应物质的神经元。这些细胞在神经纤维网中形成分支，并分布于整个中枢神经系统，约占加州海兔中枢神经元的0.5%。此外，腹神经节中的细胞通过生殖神经和鳃神经向鳃基部的外套膜底部发出突起。我们的数据表明，这个细胞网络表达单一的L11肽基因。
