Shyamala M, Fisher J M, Scheller R H
DNA. 1986 Jun;5(3):203-8. doi: 10.1089/dna.1986.5.203.
Aplysia abdominal ganglion neuron L5 is immunoreactive with an antiserum generated against the tetrapeptide Phe-Met-Arg-Phe-amide (FMRFamide); however, the specificity of this immune reagent is limited to the sequence Arg-Phe-amide. We isolated cDNA clones homologous to mRNAs specifically expressed in L5 and demonstrated that these clones do not hybridize to a previously characterized gene encoding FMRFamide. The nucleotide sequence of one of these clones, L5-67, does not encode any FMRFamide peptides but does reveal a Gly-Lys-Arg cleavage site following the amino acids Arg-Phe. This data predicts that neuron L5 expresses a peptide ending in Arg-Phe-amide, consistent with the FMRFamide immunoreactivity.
海兔腹神经节神经元L5与针对四肽苯丙氨酸-甲硫氨酸-精氨酸-苯丙氨酸酰胺（FMRF酰胺）产生的抗血清发生免疫反应；然而，这种免疫试剂的特异性仅限于精氨酸-苯丙氨酸酰胺序列。我们分离出了与在L5中特异性表达的mRNA同源的cDNA克隆，并证明这些克隆与先前鉴定的编码FMRF酰胺的基因不杂交。这些克隆之一L5-67的核苷酸序列不编码任何FMRF酰胺肽，但在精氨酸-苯丙氨酸之后确实显示出一个甘氨酸-赖氨酸-精氨酸切割位点。该数据预测神经元L5表达一种以精氨酸-苯丙氨酸酰胺结尾的肽，这与FMRF酰胺免疫反应性一致。
