Brown K A, Wolstencroft R A, Booth C G, Dumonde D C
J Immunol Methods. 1985 Oct 10;82(2):189-98. doi: 10.1016/0022-1759(85)90350-3.
Using a cell electrophoretic apparatus, which was sensitive in detecting small changes in electrophoretic mobility (EPM), the macrophage electrophoretic mobility (MEM) test was investigated as a routine method for detecting lymphokine activity. Electrophoretic analysis of guinea-pig macrophages revealed 2 main subpopulations, one with an EPM of 0.90 micron cm s-1 V-1 (fast) and the other, an EPM of 0.83 micron cm s-1 V-1 (slow). From 23 experiments the fast and slow populations were found to consist of 90% and 10% cells, respectively. When macrophages were incubated with standard guinea-pig lymphokine preparations there was a significant decrease in the fast population with a corresponding increase in the slow population. This lymphokine induced 'slowing' of the macrophages was shown to be very reproducible. Since only 50% of macrophages of high EPM were observed to respond to lymphokine activity, it is not surprising that the MEM test has failed in the past when investigators have accepted as significant a 10-15% reduction in EPM, estimated from measurements made on only 10 macrophages. Parallel bioassays indicated that there were appreciable potency differences for macrophage slowing factor (MSF) and macrophage migration inhibition factor (MIF) activities in the lymphokine preparations used which suggest that these activities may be due to different molecular entities.
使用一种对检测电泳迁移率（EPM）的微小变化敏感的细胞电泳仪，对巨噬细胞电泳迁移率（MEM）测试作为检测淋巴因子活性的常规方法进行了研究。对豚鼠巨噬细胞的电泳分析显示出两个主要亚群，一个亚群的EPM为0.90微米·厘米·秒⁻¹·伏⁻¹（快），另一个亚群的EPM为0.83微米·厘米·秒⁻¹·伏⁻¹（慢）。在23次实验中发现，快速和慢速群体分别由90%和10%的细胞组成。当巨噬细胞与标准豚鼠淋巴因子制剂一起孵育时，快速群体显著减少，慢速群体相应增加。这种淋巴因子诱导的巨噬细胞“减慢”被证明具有很高的可重复性。由于仅观察到50%的高EPM巨噬细胞对淋巴因子活性有反应，因此当研究人员过去仅根据对10个巨噬细胞的测量就将EPM降低10 - 15%视为显著时，MEM测试失败也就不足为奇了。平行生物测定表明，所使用的淋巴因子制剂中巨噬细胞减慢因子（MSF）和巨噬细胞迁移抑制因子（MIF）活性存在明显的效价差异，这表明这些活性可能归因于不同的分子实体。
