Jiangxi Province Key Laboratory of Immunology and Inflammation, Jiangxi Provincial Clinical Research Center for Laboratory Medicine, Department of Clinical Laboratory, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China.
Jiangxi Provincial Key Laboratory of Birth Defect for Prevention and Control, Medical Genetics Center, Jiangxi Maternal and Child Health Hospital, Nanchang, China.
Tissue Cell. 2024 Aug;89:102470. doi: 10.1016/j.tice.2024.102470. Epub 2024 Jul 9.
The tumor microenvironment (TME) is a highly heterogeneous ecosystem that plays critical roles in the initiation, progression, invasion, and metastasis of cancers. Extracellular vesicles (EVs), as emerging components of the host-tumor communication, are lipid-bilayer membrane structures that are secreted by most cell types into TEM and increasingly recognized as critical elements that regulate the interaction between tumor cells and their surroundings. They contain a variety of bioactive molecules, such as proteins, nucleic acids, and lipids, and participate in various pathophysiological processes while regulating intercellular communication. While many studies have focused on the EVs derived from different body fluids or cell culture supernatants, the direct isolation of tissue-derived EVs (Ti-EVs) has garnered more attention due to the advantages of tissue specificity and accurate reflection of tissue microenvironment. In this review, we summarize the protocol for isolating Ti-EVs from different tissue interstitium, discuss the role of tumor-derived and adipose tissue-derived Ti-EVs in regulating TME. In addition, we sum up the latest application of Ti-EVs as potential biomarkers for cancer diseases.
肿瘤微环境（TME）是一个高度异质的生态系统，在癌症的发生、发展、侵袭和转移中起着关键作用。细胞外囊泡（EVs）作为宿主-肿瘤通讯的新兴组成部分，是由大多数细胞类型分泌到 TEM 中的脂质双层膜结构，它们被越来越多地认为是调节肿瘤细胞与其周围环境相互作用的关键因素。它们包含多种生物活性分子，如蛋白质、核酸和脂质，并参与各种病理生理过程，同时调节细胞间通讯。虽然许多研究集中在来自不同体液或细胞培养上清液的 EVs 上，但由于组织特异性和准确反映组织微环境的优势，直接分离组织来源的 EVs（Ti-EVs）引起了更多的关注。在这篇综述中，我们总结了从不同组织间质中分离 Ti-EVs 的方案，讨论了肿瘤衍生和脂肪组织衍生的 Ti-EVs 在调节 TME 中的作用。此外，我们总结了 Ti-EVs 作为癌症疾病潜在生物标志物的最新应用。
