Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA, USA; Division of Endocrinology, Diabetes, and Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA, USA.
Cell Rep. 2024 Jul 23;43(7):114481. doi: 10.1016/j.celrep.2024.114481. Epub 2024 Jul 13.
Exercise training and cold exposure both improve systemic metabolism, but the mechanisms are not well established. Here, we tested the hypothesis that inguinal white adipose tissue (iWAT) adaptations are critical for these beneficial effects and determined the impact of exercise-trained and cold-exposed iWAT on systemic glucose metabolism and the iWAT proteome and secretome. Transplanting trained iWAT into sedentary mice improves glucose tolerance, while cold-exposed iWAT transplantation shows no such benefit. Compared to training, cold leads to more pronounced alterations in the iWAT proteome and secretome, downregulating >2,000 proteins but also boosting the thermogenic capacity of iWAT. In contrast, only training increases extracellular space and vesicle transport proteins, and only training upregulates proteins that correlate with favorable fasting glucose, suggesting fundamental changes in trained iWAT that mediate tissue-to-tissue communication. This study defines the unique exercise training- and cold exposure-induced iWAT proteomes, revealing distinct mechanisms for the beneficial effects of these interventions on metabolic health.
运动训练和冷暴露均可改善全身代谢，但具体机制尚不清楚。在这里，我们检验了一个假设，即腹股沟白色脂肪组织（iWAT）的适应性对于这些有益影响至关重要，并确定了经过运动训练和冷暴露的 iWAT 对全身葡萄糖代谢以及 iWAT 蛋白质组和分泌组的影响。将经过训练的 iWAT 移植到久坐不动的小鼠体内可改善葡萄糖耐量，而冷暴露的 iWAT 移植则没有这种益处。与训练相比，冷暴露导致 iWAT 蛋白质组和分泌组发生更明显的改变，下调超过 2000 种蛋白质，但同时也提高了 iWAT 的产热能力。相比之下，只有训练会增加细胞外空间和囊泡转运蛋白，只有训练会上调与空腹血糖相关的蛋白，这表明训练后的 iWAT 发生了根本性变化，从而介导组织间的通讯。本研究定义了独特的运动训练和冷暴露诱导的 iWAT 蛋白质组，揭示了这些干预措施对代谢健康有益影响的不同机制。
