评估大梯度强磁场对骨细胞功能影响的方案。

Protocol for evaluating the effects of large gradient high magnetic fields on osteocyte function.

机构信息

School of Life Science, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China; Research & Development Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen, Guangdong 518057, China; Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.

Research & Development Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen, Guangdong 518057, China; Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.

出版信息

STAR Protoc. 2024 Sep 20;5(3):103186. doi: 10.1016/j.xpro.2024.103186. Epub 2024 Jul 13.

DOI:10.1016/j.xpro.2024.103186

PMID:39003746

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11284420/

Abstract

Osteocytes are the main mechanosensory cells and the primary regulators of bone metabolic homeostasis. Here, we present a protocol for evaluating the effects of the large gradient high magnetic field (LG-HMF) on osteocyte function. We describe steps for establishing a corresponding cell culture system in the LG-HMF generated by a superconducting magnet. We then detail procedures for using this cell culture system to study the effects of magnetic forces on the structure and function of murine long bone osteocyte Y4 cells. For complete details on the use and execution of this protocol, please refer to Zhang et al..

摘要

成骨细胞是主要的机械敏感细胞，也是骨骼代谢稳态的主要调节者。在这里，我们提出了一种评估大梯度强磁场（LG-HMF）对成骨细胞功能影响的方案。我们描述了在超导磁体产生的 LG-HMF 中建立相应细胞培养系统的步骤。然后，我们详细介绍了使用该细胞培养系统研究磁力对鼠长骨成骨细胞 Y4 细胞结构和功能影响的过程。有关该方案使用和实施的完整详细信息，请参阅 Zhang 等人的研究。